4–4
Model 3480 Electrospray Aerosol Generator
Sucrose solutions can be made using the following method:
1.
Dissolve 1.58 grams sucrose per 10 milliliters buffer to make a
10% V/V solution.
2.
For lower concentrations of sucrose, dilute the 10% V/V
solution with buffer solution accordingly.
Ken R. Morison, Department of Chemical and Process Engineering,
University of Canterbury, Christchurch, New Zealand. “Viscosity
Equations for Sucrose Solutions: Old and New,” Paper #984
The
Asian Pacific Confederation of Chemical Engineering (APCChE)
Conference
[2002].
PSL (Polystyrene-Latex)
PSL is used to generate test aerosols in a wide size range, from
20 nm to 160 µm—the Electrospray is useful on the low end of this
range, from 20 to 100 nm. PSL is commercially available as
aqueous suspensions in 15 mL dropper bottles, and samples can
be easily made by adding one drop (50µL) of PSL to 1 mL of buffer
solution in a standard sample vial. Unlike sucrose, PSL size is
independent of concentration. Higher aerosol concentrations can be
obtained by adding more PSL, which may become necessary with
larger PSL sizes.
Proteins
Most proteins that have been used with the Electrospray range
from 3 to 12 nm in diameter. Most are commercially available from
many chemical companies. The diameters of particles generated
from proteins are independent of sample concentration. Protein
samples can be prepared using the following method:
1.
Dissolve 1 mg protein per 1 milliliter buffer solution.
2.
Dilute the 1mg/mL solution to obtain a 10 µg/mL solution.
This concentration is sufficient for most proteins when
analyzing the size distribution of the protein aerosol.
Selected proteins and their diameters are listed in Table 4-1. This is
a very limited sample of the numerous proteins available.
!
C a u t i o n
Some proteins may be dangerous to emit into the environment. Filter
dangerous aerosols before emitting them into the environment.
