4.
Run the centrifuge.
a.
Press .
The centrifuge will start, then automatically stop after 1 minute.
b.
Repeat substep 4a so that the cards are centrifuged for a total of two, consecutive, 1
‑
minute
centrifugations.
IMPORTANT!
Do not centrifuge the cards continuously for 2 minutes. The ramping up in
speed during the
two, consecutive 1
‑
minute
centrifugations is important for proper filling.
5.
Remove the cards from the centrifuge.
a.
Press .
b.
Remove the buckets from the centrifuge, then remove the card holders from the buckets.
c.
Remove each card from the card holder by lifting it gently by the card carrier sides.
6.
Examine the cards for proper filling.
When properly filled, the remaining volumes of PCR reaction mix are consistent reservoir to
reservoir.
Consistent filling
Inconsistent filling
Seal the card
The TaqMan
™
Array Card Sealer isolates the wells of an array card after it is loaded with PCR reaction
mix and centrifuged. The sealer uses a precision stylus assembly (under the carriage) to seal the main
fluid distribution channels of the array card.
Note:
In some documents, the TaqMan
™
Array Card Sealer is referred to as a Stylus Staker.
1.
Position the TaqMan
™
Array Card Sealer and its
carriage before inserting a card.
a.
Place the sealer on a benchtop that is
approximately waist
‑
high so that the carriage can
be easily maneuvered.
b.
Position the sealer with the carriage starting
position toward the front of the bench.
Ensure that the engraved arrows on the sealer
point away from you.
3
2
1
1
Carriage
2
Carriage starting position
3
Carriage ending position
Appendix C
Detailed procedures for preparation of a TaqMan
™
Array Card
Seal the card
C
206
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide