Thermo Scientific Applied Biosystems QuantStudio 6 Pro, Руководство пользователя

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4.
Pipet 100 µL of each solution into the appropriate port of the array card.
1
2
3
4
5
6
7
8
Figure 16 RNase P standards layout
1
Port 1, No Template Control
2
Port 2, Unknown A (800 copies)
3
Port 3, Unknown B (1,600 copies)
4
Port 4, Standard 200 copies
5
Port 5, Standard 400 copies
6
Port 6, Standard 800 copies
7
Port 7, Standard 1,600 copies
8
Port 8, Standard 3,200 copies
See “Load the PCR reaction mix” on page 204 .
5.
Centrifuge the array card, then check the array card for the correct fill.
See “Centrifuge the card”
on page 204 .
6.
Seal the array card.
See “Seal the card”
on page 206 .
IMPORTANT! If an array card is sealed improperly, it can leak and contaminate the sample block
and/or it can cause the associated calibration or RNase P test to fail.
7.
Run the prepared array card as soon as possible after filling it.
Perform RNase P verification
1.
In the home screen, tap (Settings)
4
Maintenance and service
4
RNase P verification .
2.
Follow the instructions on the screen to start the verification.
Chapter 7
Calibrate and verify instrument performance
Perform verification using RNase P with a TaqMan ™ Array Card
7
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QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio ™ 7 Pro Real-Time PCR System User Guide