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Anaerobic System 1-1

Thermo Scientific

Section 1

Installation and Start-Up

If the Anaerobic System is to be moved by forklift, leave the unit on the
skid until it has been moved to its designated location. A cardboard box
containing the following accessories is packed with each unit:

Outlets

Interchange

Incubator

Control
Panel

Charcoal Filter, Palladium Catalyst
and Desiccant Wafers

Figure 1-1.

Front View

Qty

Stock No.

Description

13054

Clear Plastic Tape (to secure gloves)

1 pr

188000

Glove Retaining Ring

188009

Waste Bag Holder

191022

Activated Charcoal Filter

191024

Palladium Catalyst Filter

191025

Desiccant Drying Filter

600034

375 Snapper Hose Clamp (connecting lines)

1 bx

921025

100 Clear Bags, 6” x 6” x12”

1 pr

921032

Size 9 Neoprene Gloves

285812

Plug, 15A

7285812

Instruction Sheet

380519

1/2” Hose x 1/2” FPT Adapter

Table 1-1.

Packing List

Содержание 1025

Страница 1: ...Model 1025 1029 Forma Anaerobic System Operating and Maintenance Manual 7011025 Rev 18 Visit us online to register your warranty www thermoscientific com labwarranty ...

Страница 2: ...rical schematics line cord inlet ccs 13 27418 IN 4092 7 28 11 Updated schematics to current production ccs 12 25593 SI 10224 6 30 09 BBL strips 4370504 to R65055 ccs 11 24885 SI 10078 10 30 08 Updated schematics 280005 from 290113 red LED ccs 10 24217 SI 9875 8 21 07 Added noise suppressor across inner door switch wires update schematics ccs 9 23746 6 22 07 Changed fluorescent ballast from 225329 ...

Страница 3: ... maintenance must be performed by qualified service personnel s Caution Lamps thermometers and thermoregulators contain mercury Do not put in trash Recycle or dispose as hazardous waste s Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Scientific makes no representations or warranties with respect to thi...

Страница 4: ...t that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through ...

Страница 5: ...are or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over th...

Страница 6: ...vice at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services 1 electrical service connection 2 tubing connections 3 gas regulators 4 ...

Страница 7: ...tion for Normal Operation 1 5 Connect the Foot Switch Optional 1 5 Installing the Gloves 1 5 Connect to Power 1 5 Install Equipment 1 6 Charcoal Catalyst Desiccant Wafers 1 6 Prepare the Chamber Relief Bubbler 1 7 Inspection Before Start Up 1 7 Start Up Procedure 1 8 Automatic Operation of the Interchange 1 9 Manual Operation of the Interchange 1 11 Initial Settings 1 12 Set Incubator Temperature ...

Страница 8: ...t Switch and Indicator Light 2 4 Electrical Foot Switch Optional 2 4 Vacuum Pump Outlet 2 4 Catalyst Switch and Indicating Light 2 4 Power Switch and Indicator Light 2 5 Dial Thermometer 2 5 Variable 35 C Switch Temp Control and Indicator Light 2 5 Temp Safety and Overtemp Indicator Light 2 5 Disposable Anaerobic Indicator Strips 2 6 Maintenance 3 1 Applying Preservative to Vinyl Front 3 1 Leak De...

Страница 9: ...nterchange Cycle 4 5 Replace Interchange Door Gaskets 4 6 Replace an Indicator Light 4 7 Replace the Circulation Fan Motor 4 7 Troubleshooting 5 1 Leak check Anaerobic Interchange 5 1 Leak check Anaerobic Glove Chamber 5 2 Adjustment and Part Installation 5 2 Outer Door Latch Alignment 5 2 Outer Door Hinge Alignment 5 3 Inner Door Latch Adjustment 5 3 Outer Door Switch Adjustment 5 4 Inner Door Sw...

Страница 10: ...rcoal Filter Palladium Catalyst and Desiccant Wafers Figure 1 1 Front View Qty Stock No Description 2 13054 Clear Plastic Tape to secure gloves 1 pr 188000 Glove Retaining Ring 1 188009 Waste Bag Holder 1 191022 Activated Charcoal Filter 1 191024 Palladium Catalyst Filter 1 191025 Desiccant Drying Filter 2 600034 375 Snapper Hose Clamp connecting lines 1 bx 921025 100 Clear Bags 6 x 6 x12 1 pr 921...

Страница 11: ...m system the system must meet the minimum requirements of 4 CFM capacities to 24 in Hg It is recommended that the countertop house vacuum systems be supplied with a 1 2 non tapering nipple Conventional tapered barb nipples restrict vacuum volume Caution If the system is to be connected to a house vacuum refer to Section 4 for instructions in reversing the direction of the vacuum solenoid valve bef...

Страница 12: ...ct the Vacuum Source continued Model 1025 Rear Panel anaerobic gas 10 15 psig Nylon Adapter 10 S S Hose Clamp 10 S S Hose Clamp 1 2 ID 3 4 OD Transparent Vinyl Tubing Nylon Adapter 7 2 CFM Vacuum Pump Pump Mounting Base Wooden Shelf Wooden Shelf vacuum min 4 cfm to 24 in Hg nitrogen 10 15 psig 5 amp 5 amp foot switch vacuum pump 15 amp vacuum pump only 9 amp max 1 4 20 x 3 SS Hex Head Bolt 1 4 SS ...

Страница 13: ...is recommended that the operator keep extra tanks of both gases on the premises Nitrogen A high purity grade nitrogen with a minimum purity of 99 99 maximum moisture 3 ppm is recommended Tri mixture anaerobic gas Component gases are recommended to be of a high purity grade the final gas mixture should result in less than 10 ppm O2 The Anaerobic and Nitrogen gas supply fittings are located on the p...

Страница 14: ...ce of tape from the middle of the glove cuff over the edge and half way down into the inside of the cuff Use the other three pieces of tape in the same manner equal distance around the cuff 3 Put your hand in the glove and carefully push it down through the sleeve until one third to one half of the cuff portion is protruding from the end of the sleeve 4 With the plastic tape tape the glove tightly...

Страница 15: ...mmended Electrical Loop Sterilizer SEPCO Electrostat Loop Sterilizer is recom mended Hygrometer Test Tube Racks Extra Tubes Inoculating Loops Waste Disposal Frame Bags 1 Install the wafers before start up Note The correct position of the activated wafers is stamped on the inside of the air filter door s 2 Open the air filter system door in the top center of the right side wall See illustrations 3 ...

Страница 16: ...followed correctly If the system has not been prepared correctly for operation malfunction or damage to the system may result s 1 Before starting up the system carefully check all gas connections and seal both the inner and outer doors 2 Make sure that the gloves are properly installed 3 Make sure that the charcoal catalyst and desiccant wafers are installed in the cabinet before beginning the sta...

Страница 17: ... Vacuum Nitrogen and Equalize switches to the Auto position and turn the Gas System switch On 7 Turn the Manual Fill knob fully counterclockwise Do not overturn 8 Push the Start button to cause the vacuum system to evacuate the atmospheric gas It automatically shuts off and the work chamber fills with nitrogen 9 When the chamber relief bubbler bubbles push the Start button again 10 Repeat Step 9 a...

Страница 18: ...fer cycle is factory set for three evacuations two nitrogen purges and one equalizing purge of anaerobic gas mixture The operator may alter the transfer cycle to one nitrogen purge two vacuum functions and one equalizing purge of anaerobic gas mixture Refer to Section 4 9 for instructions in making this adjustment The duration of the transfer functions may be manually controlled by setting any one...

Страница 19: ...or and remove the material 5 After removing the material be sure to secure the inner door Caution Always keep the inner door securely closed to prevent atmospheric contamination due to accidental outer door openings The outer door should also be kept closed at all times s To remove the material from the chamber 1 With the inner and outer doors secure press Start Cycle 2 When the Sequence Complete ...

Страница 20: ...ally transfer cycle during any function back to the beginning of the program 2 Put the Vacuum switch in the Manual position until the vacuum gauge reads 20 inches Then switch it back to the Auto position 3 Put the Nitrogen switch in the Manual position until the vacuum gauge drops to 2 inches Then switch it back to the Auto position 4 Repeat Steps 2 and 1 5 Repeat Step 2 again then switch the Equa...

Страница 21: ...he thermometer and set the overtemp safety thermostat as follows 1 Turn the Temp Safety control knob slowly counterclockwise until the audible alarm sounds and the overtemp indicator lights 2 Turn the overtemp control knob clockwise approximately two increments on the reference adjustment scale The alarm will be silenced and the overtemp indicator will be extinguished The overtemp safety thermosta...

Страница 22: ... Catalyst Auto Auto Auto Vacuum Nitrogen Equalize On 35 C Do Not Open Inner Door Sequence Not Complete Heat Manual Manual Manual anaerobic gas 10 15 psig nitrogen 10 15 psig 5 amp 5 amp foot switch vacuum pump only 9 amp max vacuum pump 15 amp vacuum min 4 cfm to 24 in Hg WARNING FIRE HAZARD For continued protection replace only with the same type and rating of fuse Figure 2 1 Front Control Panel ...

Страница 23: ...er relief mechanism protects the clear front panel from the strain that can be caused by excessive gas pressure A unique memory system monitors the interchange aerobic anaerobic status The two lights on the panel continually display the status of the interchange The green Sequence Complete anaerobic light indicates that the interchange is complete The interchange is in an anaerobic state the amber...

Страница 24: ...is in Manual the vacuum pump operates continuously until the switch is returned to Automatic Nitrogen Switch When the Nitrogen switch is in Manual the nitrogen solenoid permits nitrogen to continuously enter the interchange until the switch is returned to Automatic Equalize Switch When the Equalize switch is in Manual the equalize solenoid opens a tube from the work chamber to the interchange unti...

Страница 25: ...cycle starts at the beginning s The light switch controls power to the chamber light fixtures The green indicator light to the right side of the switch lights when the switch is ON The foot switch activates the electrical outlet on the far left of the chamber electrical strip An electrical outlet for the vacuum pump is mounted on the back plumbing panel of the cabinet If a portable pump is used it...

Страница 26: ...with the chamber heaters For additional information about the 35 C control potentiometer refer to Section 4 of this manual The temp safety control thermostat should be set to a temperature slightly higher than the incubator operating temperature In the event of an overtemp condition the thermostat performs two functions 1 It interrupts power to the incubator heaters 2 It activates the audible alar...

Страница 27: ...dicator foil pouches and place the strip in the desired location Refer to the instructions on the indicator strip packaging for proper usage The strip will start to turn blue at 0 01 O2 Caution Strips must be kept moist to properly indicate anaerobic condition s 2 6 Anaerobic System Thermo Scientific Section 2 Operation Disposable Anaerobic Indicator Strips ...

Страница 28: ...ial spray cleaner The glass door may be cleaned with commercial glass cleaner or with a solution of ammonia and water Clean the door gaskets routinely with a solution of mild soap and water to help prevent gas leakage It may be desirable to shut down the system remove the front and clean it thoroughly once or twice a year depending upon use To prolong the life of the vinyl front and protect it fro...

Страница 29: ...s any significant loss of gas pressure or if the mixed gas system pressure switch and solenoid valve cycles visually check the vinyl front and seal by holding your face close to the vinyl front If the leak still cannot be detected and the interchange is found to be tight the use of a electronic leak detector may become necessary Use the following method to replace a damaged glove without jeopardiz...

Страница 30: ...regulators from the gas supply cylinders to the cabinet Use a commercially available gas leak detector or a soapy water solution Inspect gloves sleeves glove sleeve connection and vinyl front for any possible leaks Before installing the gloves leak check them by filling them with water and watching for leaks Turn them inside out to dry Small holes in the vinyl front may be repaired with the clear ...

Страница 31: ...e cleaning and maintenance on a regular basis For maximum performance and efficiency it is recommended the unit be checked and calibrated periodically by a qualified service technician The following is a condensed list of preventive maintenance requirements See the specified section of the instruction manual for further details We have qualified service technicians using NIST traceable instruments...

Страница 32: ...requent regeneration may be required depending on use and environmental conditions Replace Replace the charcoal filter Every 6 months 3 7 Check gloves and sleeves for holes 9 3 1 Check and clean gaskets 9 Check wafer fan for air flow 9 Check gas injection rate 2 9 2 16 Calibrate and document dial thermometer 3 9 4 Check and document calibration of incubator 9 5 3 Check the latch handles for needed...

Страница 33: ...cedure must be performed correctly to ensure an airtight seal 2 Remove the 19 acorn nuts securing the end panel 3 Remove all the old tape gasket and Silastic from the main chamber Clean and dry the joining surface of both chambers 4 Install the tape gasket over the stud holes on the contact side of the add on chamber Make sure the gasket edges are square to ensure a tight seal The corners can be a...

Страница 34: ...temperature indicated on the thermometer If it differs from 35 C calibration is necessary Calibration is necessary following replacement of the temperature probe or the temperature controller The temperature of the incubator must be stable to correctly perform recalibration 1 Locate the 35 C controller adjustment It is the only adjustment on the front of the control panel below and to the left of ...

Страница 35: ...ands grasp the defective light bulb with the gloves and rotate the bulb away from you 1 4 turn until the bulb comes loose 3 Place the new bulb P N 225424 in the socket by reversing the above procedure If the anaerobic system is going to be used with an in house vacuum system the flow through the vacuum solenoid valve must be reversed Note the direction of the arrow on the valve body When the anaer...

Страница 36: ...l the solenoid coil to the valve body To replace the probe 1 Remove the vinyl front by extracting the screws and removing the front brace 2 Remove all cultures and equipment from the work chamber and incubator 3 Remove the two 2 nuts on the back side of the door latch holding the metal bracket 4 Remove the temperature probe from the bracket 5 Disconnect the probe cable wires noting their configura...

Страница 37: ...wise to decrease the pressure The interchange cycle is factory set at the long cycle three vacuum evacuations two nitrogen purges and one equalizing purge For a shorter cycle of two vacuum evacuations one nitrogen purge and one equalizing purge follow the steps below 1 Using a screwdriver remove the right end panel from the cabinet 2 The interchange circuit board is the large circuit board located...

Страница 38: ...lternately from top to bottom until the door exerts a slight even pressure around the outer door gasket If working with the inner door adjust the latch until the door exerts a slight even pressure around the inner door gasket 5 Carefully open the outer door and clean any excess sealant from around the new gasket When the excess sealant is removed close the outer door and latch 6 The silicone seal ...

Страница 39: ... place 7 Test the bulb then secure the panel 1 Remove the end service panel from unit 2 Remove and retain the two electrical connectors connecting the fan motor 3 Remove and retain the four screws and silicone seal securing the end panel on the circulation box Remove the panel 4 Remove the four screws and nuts securing fan motor 5 Determine the airflow direction on the new fan motor 6 Install the ...

Страница 40: ...djustments Section for the procedure Note If door adjustments do not correct the problem refer to Interchange Chamber Leaks Troubleshooting Chart in this section Repeat Steps 1 2 and 3 s 4 Depress the manual vacuum switch to achieve 20 inches of vacuum 5 Allow the unit to set a 20 inches of vacuum for 30 minutes 6 Read the vacuum gauge after 30 minutes If vacuum gauge rises 1 inch or less the unit...

Страница 41: ...k the tubing from the tank and unit 8 If the unit will not meet specification it is suggested to replace or reseal the vinyl front Note The unit can also be leak checked with an electronic leak detector if one is available s Procedures to install and or adjust various parts follow If the door is not sealing on the latch side remove the latch strike and adjust the screw on the back of the latch str...

Страница 42: ...chamber The latch is adjusted correctly when the latch pulls down firm When the latch is pulled down the door will not move from front to back Note If the latch is turned fully clockwise and the door still moves the latch bar may need to be replaced see Figure 3 s Anaerobic System 5 3 Thermo Scientific Section 5 Troubleshooting Outer Door Hinge Alignment Inner Door Latch Adjustment Loosen this scr...

Страница 43: ...ch actuation Repeat steps as needed 5 4 Anaerobic System Thermo Scientific Section 5 Troubleshooting Figure 3 Interchange Interior Door Sub assembly Hold the latch clear at the end of the slotsinthedirection of the arrow 515078 Single Roller Catch 22052 8 32 x 3 8 SS Phillips Screw 2 23080 8 SS Spring Lockwasher 2 1025 35 1 Interchange Interior Door 121036 Lift and Turn Latch 22052 8 32 x 3 8 SS P...

Страница 44: ...um solenoid leaking after pump shuts off during cycle 5 Faulty door gasket Solution 1 Adjust doors Note If outer door seal is suspected vacuum grease can be applied to gas ket for diagnostic purposes s 2 Apply RTV sealant to weld seams and pull a 20 vacuum on inter change Hold the vacuum at 20 for 5 minutes Release the vacuum and allow sealant to cure 3 Inspect fitting for defects Ensure that all ...

Страница 45: ...ump runs for a short time then shuts down gas sys tem light out Cause Solution Inner door seal is leaking Ensure inner door is pulled fully forward and latched If unsuccessful door seal or latch bar may require replacement Symptom Anaerobic gas is continuously injected into glove chamber Cause Solution Obstruction in the anaerobic gas solenoid defective gas pressure switch or manual purge valve is...

Страница 46: ...cement by quali fied technician only Symptom Unit will not maintain anaerobic state Cause Solution Air leak in the transfer chamber air leak in the glove chamber or glove chamber pressure too low front should have a slight outward bulge Symptom Interchange stuck in vacuum Cannot open inner or outer doors Cause Solution Relieve vacuum by turning manual purge knob CCW until chamber relief bottle bub...

Страница 47: ... into electrical outlet included with the system Optional Foot Switch Plugs into electrical outlet included with sys tem Exterior Dimensions Overall Cabinet 60 6 W x 30 0 H x 31 0 F B 152 9 cm x 76 2 cm x 78 7 cm Interchange Opening 10 6 W x 11 4 H 26 9 cm x 29 0 cm Incubator Interior 17 5 W x 22 0 H x 10 0 F B 42 5 cm x 55 9 cm x 25 4 cm Volume Interchange 39 liters Work Chamber 670 liters Capaci...

Страница 48: ...fm capacity to 24 in Hg minimum requirements Air Flow 1 27 CFM blower in the catalyst system Internal Outlets 4 outlets total 1 foot operated Domestic Load should not exceed 1 25 amps per outlet or 5 amps total Export Appliances should be 220V Load should not exceed 0 5 amp per outlet or 2 amps total Electrical Requirements Model 1025 90 130VAC 1 Phase 50 60Hz 14 6 FLA 120V including vacuum pump M...

Страница 49: ...icone feather gasket incubator 431500 0 30 Hg vacuum gauge 515078 Single roller catch 770001 Disposable filter 980046 1 8 FPT brass valve 120V 981024 1 2 FPT brass valve 120V 285866 10 Pin connector receptacle 285867 10 Pin connector plug 500030 Ballast universal 231192 Temp Cont 0 60C 250013 Relay DPDT 10A 120V 300109 Relay DPST N O 20A 120V 270017 T Stat B 10 Lo Temp 280004 312 Green Pilot Light...

Страница 50: ...285629 Ceramic Fuse 15 Amp 3 AB 85632 Panel Fuseholder 285813 Plug 20A 120V Hospital Grade 290027 Thermistor 1K Cable 7ft 360095 SPST Flat Black Rocker Switch 360096 SPDT MOM Blk Sq Pushbutton 940236 2 Dial Remote Bulb Thermometer 430069 12 3 SJO Cord Set 8ft 188000 Plastic Glove Retain Rings 191022 Activated Charcoal Filter 191024 Palladium Catalyst Filter 191025 Desiccant Drying Filter ...

Страница 51: ...Anaerobic System 8 1 Thermo Scientific Section 8 Electrical Schematics Electrical Schematic Model 1025 Anaerobic System 1025 70 0 D Rev 14 Page 1 of 3 ...

Страница 52: ...8 2 Anaerobic System Thermo Scientific Section 8 Electrical Schematics Electrical Schematic Model 1025 Anaerobic System 1025 70 0 D Rev 14 Page 2 of 3 ...

Страница 53: ...Anaerobic System 8 3 Thermo Scientific Section 8 Electrical Schematics Electrical Schematic Model 1025 Anaerobic System 1025 70 0 D Rev 14 Page 3 of 3 ...

Страница 54: ...8 4 Anaerobic System Thermo Scientific Section 8 Electrical Schematics Electrical Schematic Model 1029 Anaerobic System 1029 70 0 D Rev 15 Page 1 of 3 ...

Страница 55: ...Anaerobic System 8 5 Thermo Scientific Section 8 Electrical Schematics Electrical Schematic Model 1029 Anaerobic System 1029 70 0 D Rev 15 Page 2 of 3 ...

Страница 56: ...8 6 Anaerobic System Thermo Scientific Section 8 Electrical Schematics Electrical Schematic Model 1029 Anaerobic System 1029 70 0 D Rev 15 Page 3 of 3 ...

Страница 57: ...arranty shall not extend the warranty to either the equipment or to the compo nent part beyond the original warranty period The Technical Services Department must give prior approval for return of any components or equipment At Thermo s option all non conforming parts must be returned to Thermo Fisher Scientific postage paid and replacement parts are shipped FOB desti nation THIS WARRANTY IS EXCLU...

Страница 58: ...r to performance of any repairs Expendable items glass fil ters reagents tubing and gaskets are excluded from this warranty Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component part beyond the original warranty period The Technical Services Department must give prior approval for return of any com ponen...

Страница 59: ...her Scientific and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific Asheville LLC 401 Millcreek Road Marietta Ohio 45750 United States A Thermo Fisher Scientific Brand ...

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