PerkinElmer LS 55, Руководство пользователя

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Accessories: Installation and Use . 53
Stirred Semi-micro Cuvette
Figure 34 The stirred semi-micro cuvette
Description
Micro and semi-micro cuvettes, which allow the user to measure smaller sample
volumes than standard 1cm pathlength cuvettes, typically have disadvantages in
that stirring and thermostatting are difficult to achieve.
In the case of stirring, this is either because the internal dimensions of the
microcuvette are too small or because the small size of the stirrer for cuvettes with
5 mm pathlength do not function efficiently.
Thermostatting is difficult due to the use of a semi-micro cuvette adaptor, which
fits into the standard cuvette holder and accepts the smaller cuvette. These
adaptors are normally manufactured from polymer material, which is an effective
thermal insulator.
The stirred semi-micro cuvette (B0631132) overcomes both of these problems:
• The outside dimensions are the same as for a 1 cm cuvette, giving good
contact with the cellholder for thermostatting (as detailed in
Installing
Thermostatting Tubing to Cellholders
on page 45), but the internal dimensions
are smaller for decreased sample volume.
• At the base of the cuvette there is a chamber for insertion of a standard stirrer.