Labfors 5 – Version for Microorganisms
Fermentation/Cultivation
22 January 2019
Page
157
of
205
9.5 Emptying the Culture Vessel
Depending on the user specifications, the culture vessel can be
emptied either before or after autoclaving.
A previously emptied and culture vessel filled only with water for
autoclaving is easier to clean afterwards.
For emptying the culture vessel, the same options as for harvesting
are available. For more information, see the “Harvest” section.
If the culture will not be used further, it must be inactivated accord-
ing to the current in-house instructions (e.g. by autoclaving or by
lowering the pH value), and subsequently disposed of in an envi-
ronmentally sound manner according to the local regulations.
9.6 Emptying the Reagent Hoses
Before autoclaving the culture vessel with accessories, all reagent
hoses must be completely emptied using the corresponding pump.
This can either be done manually and individually for each pump
using the rocker switch on the basic unit or all pumps are emptied
time-controlled at once via the operating unit.
It is also recommended to thoroughly rinse the hoses with water
after emptying and before autoclaving.
For details about automatic emptying of all pump hoses refer to the
separate operating manual of the touch screen software.
9.7 Switching off the Equipment
When the harvest is finished or the culture vessel has been emp-
tied and the reagent hoses are also empty, the equipment can be
switched off.
Proceed as follows:
Procedure
1.
Ensure that the bioreactor has been stopped. If necessary,
stop it in the touch screen software on the operating panel
.
2.
Shutdown the system on the operating panel.
3.
Switch off the equipment at the main switch.
Содержание Labfors 5
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