Infors HT Labfors 5, Руководство по эксплуатации

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Labfors 5 – Version for Microorganisms
Fermentation/Cultivation
22 January 2019 Page 149 of 205
9 Fermentation/Cultivation
The following sections describe the work necessary for the perfor-
mance of and after the completion of a fermentation/cultivation,
before the culture vessel with accessories is thoroughly cleaned
and then prepared for another cultivation.
This essentially comprises:

Preparing the medium, starting the bioreactor

Sampling

Inoculation

Harvest

Stopping the bioreactor, if necessary emptying the vessel

Autoclaving the culture vessel and accessories
The requirement for the first item is that the culture vessel and ac-
cessories are autoclaved, cooled and connected to the basic unit.
All cable and hose connections between the equipment and the
culture vessel, including the reagent bottles, are present, pump
heads are mounted and the reagent hoses are filled. Depending on
the user specifications, the pO
2
sensor is already calibrated.
9.1 Preparing the Medium
Before the first sampling, which usually takes place as a ‘zero sam-
ple’ before inoculation, and before the inoculation itself, the me-
dium must be warmed to the desired temperature. If necessary, the
pO
2
concentration and the pH are set. The time required for this
depends on the working volume.
Set and activate the desired setpoint of the parameters in question
on the operating panel, and start the bioreactor.
Depending on the specifications defined by the user, the pO
2
sen-
sor is calibrated either before the vessel is filled with medium or
afterwards, in the prepared medium.