Labfors 5 – Version for Microorganisms
Fermentation/Cultivation
Page
158
of
205
22 January 2019
ATTENTION
Switching the equipment off at the main switch without previ-
ously stopping the bioreactor and shutting down the system on
the operating panel may lead to damage of the operating panel!
4.
Close the supply lines (water, gas).
5.
Let the motor cool down.
6.
Autoclave the vessel, component parts and accessories as
per the user-specific specifications and then clean them.
9.8 Autoclaving the Culture Vessel After Fermentation/Cultivation
After emptying the culture vessel and before cleaning, the culture
vessel must be autoclaved with all accessories.
When doing so, do not autoclave the culture vessel when com-
pletely dry and observe the same safety regulations as when auto-
claving before cultivation.
Before starting, ensure:
There is liquid in the culture vessel (autoclavable medium or
approx. 10 mL water per litre working volume).
Reagents and feed solution have been pumped back out of
the hoses.
The equipment is switched off.
The motor has cooled down.
Proceed as follows to prepare the culture vessel and accessories
for autoclaving after fermentation/cultivation:
Procedure
1.
Clamp off the hoses of the reagent bottles.
2.
Clamp off the hose of the sparger.
3.
Remove all cable and hose connections between the basic
unit and the culture vessel:
a) Uncouple the motor and place it to the side.
b) Unplug the sensor cables.
c) Pull the temperature sensor out of the pocket.
d) If an exit gas cooler is in use: disconnect the water inlet
and water outlet hoses from the exit gas cooler.
e) Depending on the temperature control system:
Содержание Labfors 5
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