Infors HT Labfors 5, Руководство по эксплуатации

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Labfors 5 – Version for Microorganisms
Fermentation/Cultivation
22 January 2019 Page 155 of 205
6. Immediately pierce the inoculation needle through the septum.
7.
Screw the inoculation needle into the septum collar.
8.
Transfer the desired volume of inoculum into the culture ves-
sel.
9.
Clamp off the silicone hose.
Or: Pull the inoculation needle out and close the septum collar
with the blanking plug. But this method is not secure regarding
preventing contamination.
9.3.2 Inoculation with a Syringe
Proceed as follows for the inoculation:
Procedure 1. Fill the syringe with the required amount of inoculum.
2. Unscrew the blanking plug from the septum collar.
As a possible additional protection against contamination:
Before piercing, drop a few drops of ethanol (70 %) on the
septum.
3. Pierce the septum and inject the inoculum.
4. Remove the needle from the septum and close the septum
collar with a blanking plug.
9.3.3 Inoculation Using Dip Tube / Addition Port Adapter
Proceed as follows during inoculation:
Procedure
1.
Fill the inoculum under sterile conditions into the prepared
container.
2.
Create a sterile hose connection with the dip tube/addition
port adapter.
3. Transfer the desired volume of inoculum into the culture ves-
sel. Pump it, if necessary.
4. Clamp off the hose with the hose clamp, weld it if necessary.