Labfors 5 – Version for Microorganisms
Before Fermentation/Cultivation
22 January 2019
Page
147
of
205
2.
Plug the cable connector onto the sensor.
3.
Turn the bajonet cap of the sensor cable clockwise und push
gently towards the sensor.
Ensure the cable is not twisted or buckled.
ATTENTION
The cable shield can be damaged by buckling or twisting. This
may lead to faulty measurements.
HAMILTON pO2 sensor
To connect the HAMILTON pO
2
sensor, proceed in the same man-
ner as with the HAMILTON pH sensor; see the chapter “Connect-
ing the pH Sensor”, section “HAMILTON pH sensor”.
8.2.13 Polarising the pO
2
Sensor (METTLER)
Polarographic pO
2
sensors must be polarised at initial operation or
after disconnection from the voltage source. Correct calibration is
not possible otherwise. This means that the pO
2
sensor must be
polarised before calibration.
For polarisation, the sensor cable must simply be connected to the
pO
2
sensor and the equipment must be switched on at the main
switch.
Duration of polarisation (= polarisation time) depends on how long
the pO
2
sensor has been disconnected from the voltage source (=
depolarisation time)
As a general rule: if depolarisation time > 30 minutes, the minimum
polarisation time is 360 minutes.
More details about polarisation can be found in the separate docu-
mentation form the manufacturer METTLER TOLEDO.
8.2.14 Calibrating the pO
2
Sensor
Generally speaking, the following applies: The pO
2
sensor should
be calibrated after autoclaving has been performed because the
sterilisation process may change the steepness of the pO
2
sensor.
As a rule, a 1-point calibration to 100 % is usually sufficient for ex-
act measurement, and should be carried out before each cultiva-
tion.
Depending on the specifications defined by the user, the pO
2
sen-
sor is calibrated either before the vessel is filled with medium or
afterwards, in the prepared medium.
Содержание Labfors 5
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