Ch 3 – Preparing to Take a Picture
Image Setup Options
User’s Guide to the SPOT Cooled Color Digital Camera, 6/9/98
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When you open an image setup, the Image Setup screen displays the default white
balance values for that Setup, which are the values that were last saved to the Image
Setup. If you capture an image without computing a new white balance, SPOT uses the
default values.
To compute new white balance values for an image setup, follow these steps:
1.
Set the illumination and the objective that you will use for the image capture.
2.
Show the camera a sample of white light. This step varies according to microscopy
technique and sample type:
−
For
transmitted light brightfield microscopy
, position the slide so that the
specimen is not in the field of view, but light is going through the slide near the
specimen.
−
For
brightfield metallurgical microscopy
, replace your sample with a mirror.
−
For
low magnification work
using an EPI-illuminator (shining a light on the top
of your specimen from off to one side), replace your sample with a white sheet of
paper.
!
Note: EPI illumination is usually performed with a stereo microscope or a macro
lens.
3.
From the Camera menu, select the Compute White Balance Values option, or use the
toolbar and click on the Compute White Balance Values button, illustrated here:
4.
The Compute White Balance window appears, as follows:
5.
Click on the Begin button to start the calculation. The Compute White Balance
window displays the status message
Computing white balance values
. You should
hear a series of clicks as the camera samples the light.
