IMPORTANT!
Wear powder-free gloves while creating the dye plate.
1.
Prepare 2 mL of the custom dye at the concentration determined in “Determine
optimum dye concentration“ on page 153.
2.
Pipet 20 µL of the diluted custom dye to all wells of an optical reaction plate.
3.
Seal the wells of the reaction plate using an optical adhesive cover.
4.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Note:
The custom dye calibration plate must be well mixed and centrifuged.
5.
Verify that the liquid in each well of the plate is at the bottom of the well. If not,
centrifuge the plate again at a higher rpm and for a longer period of time.
1.
Start the dye calibration:
a.
In the Home screen of the QuantStudio
™
Real-Time PCR Software, click
Instrument Console.
b.
In the Instrument Console, select your system from the list of instruments
on the network, then click Add to My Instruments.
c.
Select the system, then click Manage Instrument.
d.
In the Instrument Manager, click Maintenance, then click Dye.
e.
In the Background Calibration screen, click Start Calibration.
2.
In the Dye window, select a custom dye from the list or create the custom dye:
a.
Click New Dye.
b.
In the Dye Library dialog box, click New.
Create a custom
dye plate
Add the custom
dye to the
software
Appendix F
Calibration consumable preparation
Create a custom dye plate for calibration
F
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
155
