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Method for determining absolute target quantity in samples. With the standard curve
method, the software measures amplification of the target in samples and in a
standard dilution series. Data from the standard dilution series are used to generate
the standard curve. Using the standard curve, the software interpolates the absolute
quantity of target in the samples.
See also “standard“ on page 191 and “standard curve“ on page 191.
In standard curve and relative standard curve experiments, a set of standards
containing a range of known quantities. The standard dilution series is prepared by
serially diluting standards. For example, the standard stock is used to prepare the first
dilution point, the first dilution point is used to prepare the second dilution point, and
so on. In the software, the volumes needed to prepare a standard dilution series are
calculated by the number of dilution points, the number of standard replicates, the
starting quantity, the serial factor, and the standard concentration in the stock. See
also “standard curve“ on page 191.
In the PCR reaction, a known quantity. In standard curve experiments, the quantity of
target in the standard. From the software, the units for standard quantity can be for
mass, copy number, viral load, or other units for measuring the quantity of target.
Standard quantity can also refer to dilution factor.
When defining a standard curve in the software, the highest quantity.
A component of the thermal profile. For each step in the thermal profile, you can set
the ramp rate (ramp increment for melt curve steps), hold temperature, and hold time
(duration) are set as part of the .tdd file. Data collection can also be turned on or off
for the ramp or the hold parts of the step in each .tdd file. For cycling stages, a step is
also defined by the AutoDelta status.
Dye supplied by Thermo Fisher Scientific and calibrated on the QuantStudio
™
6 and 7
Flex Systems. Before you use system dyes in your experiments, make sure the dye
calibration is current in the Instrument Console.
The nucleic acid sequence to amplify and detect.
From the software, a color assigned to a target to identify the target in the plate layout
and analysis plots.
From the software, the type of reaction performed in the well for the target or SNP
assay.
Wells containing identical reaction components, including sample; important for
evaluating precision.
From the software, a display of temperatures for the instrument cover and instrument
block during the instrument run.
The type of nucleic acid to add to the PCR reaction.
standard curve
method
standard dilution
series
standard quantity
starting quantity
step
system dye
target
target color
task
technical
replicates
temperature plot
template
Limited product warranty
192
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide