A tab-delimited file (.txt) or Microsoft
™
Excel
™
file (.xls or .xlsx) that contains the
sample name and custom sample properties for a single experiment.
A separately licensed software module that provides:
• System Security – Controls user access to the software. Provides a default
Administrator user account. You can define additional user accounts and
permissions.
• Auditing – Tracks changes made to library items, actions performed by users,
and changes to the Security and Audit settings. The software automatically audits
some actions silently. You can select other items for auditing and specify the audit
mode. Provides reports for audited library items, Security and Audit changes,
and actions.
• Electronic Signature (e-signature) – Controls whether users are permitted,
prompted, or required to provide a user name and password when accessing
certain software features. You can select which features are controlled and the
number of signatures required for access. When authorized persons use this
feature, they are creating a legally binding signature.
In the software, a numeric value that defines the sequence of quantities in the
standard curve. The serial factor and the starting quantity are used to calculate the
standard quantity for each point in the standard curve. For example, if the standard
curve is defined with a serial factor of 1:10 or 10X, the difference between any two
adjacent points in the curve is 10-fold.
Regression coefficient calculated from the regression line in the standard curve. The
slope indicates the PCR amplification efficiency for the assay. A slope of -3.32
indicates 100% amplification efficiency.
See also “amplification efficiency (EFF%)“ on page 184 and “regression line“ on
In the thermal profile, a group of one or more steps. Examples: PCR stage, cycling
stage (also called amplification stage), and hold stage.
A sample that you dilute and amplify along with unknown samples. This dilution
series can contain known starting quantities of the target of interest (absolute
standard curve) or it can be of known dilution factor (relative standard curve).
Following the run, the software interpolates the C
t
values of the unknowns to this
curve, yielding either specific quantities of the target (for absolute curves) or relative
quantities (for relative dilution curves).
See also “standard curve“ on page 191.
In standard curve and relative standard curve experiments:
• The best-fit line in a plot of the C
t
values from the standard reactions plotted
against standard quantities. See also “regression line“ on page 190.
• A set of standards containing a range of known quantities. Results from the
standard curve reactions are used to generate the standard curve. The standard
curve is defined by the number of points in the dilution series, the number of
standard replicates, the starting quantity, and the serial factor.
sample definition
file
security, auditing
and e-signature
serial factor
slope
stage
standard
standard curve
Limited product warranty
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
191
