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b.
Place the filled carrier clips into the centrifuge buckets. Make sure that the
array card fill reservoirs and bucket and clip labels face outward when
loaded into the centrifuge.
IMPORTANT!
You must run the centrifuge with all four buckets in place
and each of the two carriers filled with array cards. Place empty array cards
into unfilled slots.
IMPORTANT!
Balance the loads in opposite buckets in the centrifuge.
c.
Close the centrifuge cover, then spin the array card(s) for 1 minute at
1200 rpm.
d.
When the run is finished, stop the centrifuge, then spin the array card(s)
again for 1 minute at 1200 rpm.
IMPORTANT!
Do not try to save time by doing one spin for 2 minutes. The
two sets of ramps are important for a good fill into the array card.
7.
When the second run is finished, open the centrifuge and check that the fluid
levels in the reservoirs of each array card have decreased by the same amount.
Also, check all wells for bubbles and note the locations as possible problems.
Correct fill
Incorrect/partial fill
If necessary, centrifuge the array cards for an additional minute to fill any
unfilled wells. Do not exceed three 1-minute runs or centrifuge the array card for
longer than 1 minute at a time.
Chapter 2
Calibrate the instrument
Preparing array cards for instrument calibration
2
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6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
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