2 Instrument Configuration
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the aperture, allowing them to be counted according to the number of pulses emitted.
2.2.5.2 Measurement Process
The measurement process for the RBC/PLT measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the RBC/PLT bath as the base
liquid, before using the sample probe to aspirate the sample diluted for the first time from the
WBC bath into the RBC/PLT bath.
2. Mixing: Open Valve LV16 to generate air bubbles through the intermittent valve opening to mix
the sample well.
3. Measurement: Open Valve LV18 to aspirate the sample out of the RBC/PLT bath through the
aperture by means of the negative-pressure chamber (with a negative pressure of -30Kpa). The
RBC and PLT particles generate electric pulses of various amplitudes when travelling through
the aperture, allowing the RBC and PLT cells to be counted according to the number of pulses
emitted.
4. Cleaning: To clean, open Valve LV02 and add the diluent into the RBC/PLT bath using the diluent
syringe.
5. Waste discharge: Waste is discharged by opening Valve LV13, LV15 and Pump P1.
2.2.6 Sampling and Blood-dispensing Module
This module consists of the sample probe, the sampling syringe, the sampling tube, the swab, the
horizontal motor, and the vertical motor.
This is how the sampling and blood-dispensing module works:
Open LV04 and aspirate the sample from the testing tube using the sampling
syringe→Clean the
outer wall of the sample probe→ …until it reaches the WBC bath→Put aside a fixed amount of blood
in the WBC bath→Clean the outer and inner wall of the sample probe→Aspirate out of the WBC bath
a fixed amount of the sample that has been d
iluted once→Clean the outer wall of the sample probe
and move the sample probe to the RBC bath→Add into the RBC bath the sample that has been
diluted once→Clean the outer wall of the sample probe to complete the entire process of sampling
and blood dispensing.
In summary, this module works by aspirating the sample and dispensing a fixed amount of the
sample into the corresponding reaction baths, while cleaning the inner and outer walls of the sample
probe to prevent cross-contamination.
2.2.7 Power Supply and Waste Discharge Module
⚫
Power supply module
By opening LV15 and Pump P1 to establish a negative pressure of -30Kpa, this module provides
propulsion for the counting of the impedance channel.
⚫
Waste disposal module
This module consists of the following parts:
➢
Discharge of waste resulting from swab cleansing
Pump P1 is opened to discharge any waste resulting from using the swab to clean the
sample probe.
