2 Instrument Configuration
Copyright © WOODLEY EQUIPMENT COMPANY LTD.
11
2.2.2 Hydraulics Diagram for the Overall Unit
Figure 2-8 Hydraulics diagram for the overall unit
RBC
WBC
LV3
LV2
LV1
LV4
LV5
LV7
LV9
LV11
LV12
LV15
LV17
LV18
LV6
LV19
SE Sampling
Syringe
Lyse Syringe
250ul 10ml
2.5ml
10ml
2.5ml
J13
T70
T29
T29
T29
J11
J12
T70
T70
T20 T134
T9
J6
J7
J6
J6
J6
J6
J6
T29
J6
J2
T29
T29
T29
T29
T29
J6
J6
T70
Check
valve
T72
T72
J5
J5
T72
J5
J2
T80
T80
T29
T29
T70
T80
T10
T10
T70
T48
T80
T48
T29
J3
J4
T11
T11
T14
J9
P1
T70
T67
T67
T70
One-way
valve
Air Filter
DIFF I
LH
DILU
LH
T237
V5 LY2
T115
T70
V5 LY1
T13
T70
DIFF I
T214
V5 DIL
T15
T14
J1
T70
J3
P2
T80
T29
T70
J3
T80
T80
T80
T80
J3
T70
T171
J4
T29
T69
T28
T211
J3
T80
T80
T168
T29
T164
T197
T165
T80
T29
J3
T29
T29
J8
T105
LV16
LV8
J8
J2
J2
T5
T65
T58
T65
T52
T52
T51
T204
T169
T33
T42
T158
T238
T40
T80
T21
T156
T240
T242
T65
T236
T150
T152
T162
T66
T83
T197
T59
T214
T221
T210
T210
T70
T70
T70
T70
T72
T72
T72
T171
T168
T177
T197
T186
T195
T72
T72
T80
T80
T80
T80
T176
T70
250ul
T80
J3
T80
J3
Negative-
pressure
Sensor
Positive-pressure Sensor
Dual Pipe
LV14
LV13
LV10
LV20
LV21
T29
T19
T29
T29
T29
J3
T80
T195
J10
T80
T80
T65
J2
J2
T197
J10
T73
T39
T82
T87
T67
T67
T80
T89
J10
T171
T38
T239
T171
T170
T177
T199
J10
J10
T170
T57
T105
T241
J6
J2
T70
J10
T80
J6
2
3 1
2:COM
1:NC
3:NO
Three-way valve
Tow-way valve
2
1
Note
:
LV-liquid value
;
P-pump
;
T-pipe
;
J-connector
Name
Quantity
Note
Valve
Pump
Syring
21
6
2
2:IN
1:OUT
J3
T43
T26
T65
2.2.3 DIFF/WBC Measurement Channel
2.2.3.1 Channel Principles and a Description of Their Functions
⚫
Measurement principles: Flow cytometry and scattering emission of the semiconductor laser
⚫
Measurement parameters: WBC, Mon#, Mon%, Lym#, Lym%, Neu#, Neu%, Eos#, Eos%, Bas#,
Bas%
⚫
Graph information: DIFF 2D scattergram and BASO 2D scattergram.
⚫
Reagents for use
➢
V5 LY1 Lyse: Lyses the RBC and combines with haemoglobin.
➢
V5 LY2: Lyses the RBCs and specifically treats different types of WBCs.
➢
V5 DIL Diluent: This diluting and cleaning agent can provide an electrically conductive
environment and process cells and so on in bulk.
⚫
Function description
➢
DIFF parameters measurement
The diluted sample is mixed well for a full reaction with V5 LY2 Lyse in the WBC bath. Then
the sample is placed between the flow chamber inlet and the sampling syringe; meanwhile,
the sheath flow is activated and kept steady so that it can hold the sample in motion until it