86
03-914648-00:7
The following are typical conditions for carrying out an on-column injection.
The injector should be maintained at 10 - 20 °C below the solvent boiling
point at injection. In this example the solvent is hexane which has a boiling
point of 68
°
C.
Note:
With the high performance insert the taper is
towards the bottom
of the
injector. With the on-column insert (530 µm) the taper is
towards the top
of the
injector.
Table 12 On-Column Injection
Injector Insert
Install either the high performance tapered insert (P/N 01-900109-06)
for narrow to wide bore columns or the on column insert (P/N 01-
900109-07) for large bore (0.53 mm ID) columns.
Seal/Position Column
Seal the column within the tapered insert.
Injector Temperature
Initial temperature 50
°
C, hold for 0.1 minute, ramp to 250
°
C at
150
°
C/minute, hold 10 minutes.
Column Temperature
50
°
C initial for 2 min, ramp to 250
°
C at 20
°
C/min, hold 5 minutes.
Carrier gas control
Split does not apply in on-column mode. The Splitless vent flow must
be set to 20 ml/min or greater in Setup. Delete any splitter program and
set initial state to off in the Injector section.
Large
Volume
Injection
The large volume injection technique is used where the absolute lowest level
of detection is required. Up to 100 µL of sample may be introduced into the
1079 injector using a 100 µL syringe (03-925414-01). Note that if this
technique needs to be automated using the 8200 AutoSampler a Large
Volume Injection kit should be ordered (03-925413-91). The sample is
injected at a very slow rate while the injector temperature is set a few
degrees below the solvent boiling point. In the example below hexane is
used as the solvent which has a boiling point of 68
°
C.
Using the large volume injection technique the injector is maintained in the
split ON state at the beginning of the run to vent most of the solvent. The
sample components are trapped in the injector insert so the same type of
insert is used as for temperature ramped splitless injection. The split state is
then programmed to OFF and the injector temperature ramped to transfer
the sample components to the head of the column. The following are typical
conditions for a large volume injection.
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