58
03-914648-00:7
Tools and
equipment
needed
•
Ceramic scoring wafer (01-900158-00) or a scoring tool
•
Magnifying lens (00-997369-00)
Follow these steps to prepare the end of the column for insertion into the
1177 Injector.
1
Slide the capillary column nut over the fused silica column end.
2
Install the appropriate ferrule over the column end. See
above table.
Make sure that the tapered end of the ferrule is toward the inside of
the column nut.
3
Use the scoring tool to score the column once lightly about 1 to 2 cm
from the end of the column.
4
With your thumbs and forefingers, grasp the column on each side of
the score and bend the column carefully away from the score mark
until it breaks at the score mark.
5
Examine the column with a magnifying lens to determine whether it
was properly cut.
Follow these steps to install the chromatographic column into the 1177
Injector.
Note:
This procedure is for installation in an injector that is equipped with an
open style insert, e.g., the split, splitless or temperature ramped splitless modes.
1
Set the column oven temperature to 50
°
C and wait for it to cool to
this temperature. Turn the column oven power off by pressing the
“Turn Oven Off” softkey. Cool the injector to 70
°
C or lower.
WARNING:
BURN HAZARD
2
The column should extend 3.7 cm from bottom of the column nut.
In the following procedure, you will mark the 3.7 cm insertion depth
on the column and then adjust the nut and ferrule so that 3.7 cm
extends beyond the column nut. Move the nut and ferrule to within
3 cm of the column end. Measure 3.7 cm from the end of the
column and mark this distance with a felt pen or typewriter
correction fluid or a small piece of tape.
Note:
DO NOT allow the column nut and ferrule to fall past the
mark because the ink or correction fluid can contaminate the
ferrule.
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