Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

Page: 81 / 148

5 • Installation
UHPLC System
Operating Manual Page 81
5.8 Flushing the System
This section describes how to flush the Vanquish system after
installation. As certain system components are filled with isopropanol
during shipment, flushing is required before the system can be
operated.
Flushing the System without Column
Follow these steps:
NOTICE
To avoid damage to other modules in the Vanquish system, verify the
following before you begin:
• The detector is not connected to the flow path.
• No column is installed in the column compartment.
1. Purge the pump.
2. Have the pump deliver the solvent used for purging for a short time.
Flushing the System with Column Installed
Flush the system again for a short time after connecting the column with
a solvent suitable for your application. Follow these steps:
1. Verify that the detector is not connected to the flow path.
2. Have the pump deliver the solvent for a short time. Observe the
pressure limit and other properties of the column.
Flushing the System with Column and Flow Cell Installed
Flush the system again for a short time after connecting the flow cell
with a solvent suitable for your application. Observe the guidelines for
flow cells in the
Operating Instructions of the detector.
NOTICE
• When the charged aerosol detector is connected in the system flow
path, flush the detector only when the detector gas flow is turned
on.
• Flush the detector with flow from the pump.