Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

Page: 115 / 148

8 • Troubleshooting
UHPLC System
Operating Manual Page 115
8.2 System Troubleshooting
This section is a guide to troubleshooting issues that may arise during
operation of the Vanquish system. Locate the table for the type of
symptom you have, find the possible cause, and use the description of
the solution to help you solve your problem quickly.
TIP
For information on troubleshooting issues that may arise during
operation with the Vanquish charged aerosol detector and the Vanquish
fluorescence detector, refer to the respective
Operating Manual for the
detector.
Note that this section provides information on symptoms and causes
directly related to the Vanquish system modules and connections
between the modules only. For information about general chromato-
graphy and application troubleshooting, refer to the technical literature.
8.2.1 Peak Shape
Peak tailing
Possible Cause Remedial Action
Extra column volume too
large Use short capillary connections with a suitable inner diameter. Use appropriate
Viper capillaries.
Improper capillary
connections Check fittings for correct placement. Loosen and retighten Viper fittings (refer to
the
Viper Installation and Operation Guide ). Use only the appropriate fittings (see
Connecting Fittings, Capillaries, and Tubing ( } page 68) ).
Peak broadening
Possible Cause Remedial Action
Flow cell volume too
large Use a smaller volume flow cell.
Extra-column volume
too large • If early peaks are broader than later eluting ones, check the capillary I.D. and
length, sample loop size, flow cell, for example.
• Use a capillary with a smaller volume for the connection between column
compartment and detector.
Temperature gradient
in column • Use a pre-heater.
• If forced air mode is used, consider switching to still air mode (refer to Column
Compartment Operating Manual ).
Detector response time
too long and/or data
collection rate too low Select a suitable response time and/or data collection rate.