6
•
Operation
UHPLC System
Operating Manual
Page 97
6.7
Shutting Down the System
If the system will not be operated for some time, observe the general
guidelines outlined below.
Interrupting Operation of the System for a Short Period
To interrupt operation of the system for a short period (short-term
shutdown), for example, overnight, observe these guidelines for the
Vanquish system modules, as required by your system arrangement:
•
For your Vanquish detector, note the following:
Detector Type
Description
Charged aerosol
detector
Check that sufficient gas is available to continue gas flowing
through the detector. This is to prevent any build-up of
residue from solvents or analytes. Gas must be flowing when
pump flow is delivered to the detector.
UV/VIS detectors
The lamp(s) in the detector can remain turned on.
Variable wavelength detector and VH-D10 diode array
detector only:
The shutter can be moved to a closed position
for protection of the flow cell.
Fluorescence
detector
Turn off temperature control for the flow cell.
•
Apply a flow of 0.05 mL/min and have the pump deliver an
appropriate solvent.
Check the lower pressure limit for the pump and adapt the value if
necessary. If the pressure falls below the lower limit, the pump stops
the flow.
•
Set the injection valve in the autosampler to the Inject position.
•
Make sure that the temperature of the column does not exceed
40 °C.
•
When resuming operation, let the flow equilibrate and verify that
the operating parameters for the other system modules are set as
required before proceeding.
TIP
The Chromeleon software provides procedures for automatically
preparing the chromatography system for shutdown. The procedures
include, for example, operations for reducing the flow rate, reducing the
temperature in temperature-controlled devices, and turning off the
detector lamps. For information about
Smart Shutdown
and
Smart
Standby
, refer to the
Chromeleon Help
.
Summary of Contents for thermoscientific Vanquish UHPLC System
Page 4: ...Contacting Us Page 4 UHPLC System Operating Manual ...
Page 28: ...2 Safety Page 28 UHPLC System Operating Manual ...
Page 34: ...3 System Overview Page 34 UHPLC System Operating Manual ...
Page 38: ...4 Unpacking Page 38 UHPLC System Operating Manual ...
Page 82: ...5 Installation Page 82 UHPLC System Operating Manual ...
Page 100: ...6 Operation Page 100 UHPLC System Operating Manual ...
Page 112: ...7 Maintenance and Service Page 112 UHPLC System Operating Manual ...
Page 128: ...8 Troubleshooting Page 128 UHPLC System Operating Manual ...
Page 132: ...9 Specifications Page 132 UHPLC System Operating Manual ...
Page 144: ...11 Appendix Page 144 UHPLC System Operating Manual ...