Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

Page: 87 / 148

6 • Operation
UHPLC System
Operating Manual Page 87
6.4 Preparing the System for Operation
This section gives information on any additional steps that are required
to prepare the system for operation and sample analysis.
Before Operating the System for the First Time
Prepare the system for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for
the first time:
• When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path.
Follow the instructions in
Flushing the System ( } page 81) .
• Some components of the system are filled with isopropanol when
the system is shipped from the manufacturing site. When operating
the system for the first time, use solvents that are miscible with
isopropanol. If they are not, use an appropriate intermediate
solvent.
• Verify that any air bubbles are completely flushed out of the system
flow path.
Refer to the Operating Manual for each system module for any steps
that may be required before initial operation.
Before Starting Sample Analysis
Before starting an analysis:
• Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
• Verify that the doors of all modules in the Vanquish system are
closed.
• Make sure that the chromatography system is properly equilibrated
(see further down).