Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

Page: 88 / 148

6 • Operation
Page 88 UHPLC System
Operating Manual
System Equilibration
System equilibration should include the following operations:
• Purging the pump ( all channels, including those not used for the
application)
• Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run
• Warming up (or cooling down) all temperature-controlled devices in
the system to the starting temperature. Temperature-controlled
devices can be, for example
¨ Column compartment and post-column cooler
¨ Sample compartment thermostatting in the autosampler
¨
Flow cell in a fluorescence detector
¨
Evaporation tube in a charged aerosol detector
• Turning on the lamp (or lamps) in the UV/VIS detector
• Monitoring the pump pressure and pressure ripple and checking
that the pressure is stable and the ripple within reasonable limits for
the application
• Monitoring the detector signal and checking whether the detector
signal is stable so that the drift and signal noise are within
reasonable limits for the application
• Performing an autozero of the detector baseline
TIP The Chromeleon software supports procedures for automatically
starting a chromatography system in the software ( Smart Startup ). The
startup procedure includes the operations for system equilibration. For
details, refer to the
Chromeleon Help .