Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

Page: 90 / 148

6 • Operation
Page 90 UHPLC System
Operating Manual
• The pumps are shipped with the following piston seals:
Pump Piston Seals
VC‑pumps except
VC‑P21 and VC‑P33 The pumps are shipped with UHMW-PE piston seals. Using
tetrahydrofuran, ketones, or ammonium hydroxide as
solvents can damage the seals.
Under harsh conditions, consider replacing the UHMW-PE
piston seals with carbon-fiber filled PTFE piston seals (part
no. 6040.0306). In this case, also install new pistons.
Exchanging the piston seals but keeping the pistons that
were used with the UHMW-PE piston seals may impair the
seal life of the PTFE seals. In addition, note that slightly
increased abrasion may be observed with these seals,
depending on the application. Consider testing the
permeability of the static mixer regularly at short intervals.
VC‑P21 and VC‑P33
pumps The pumps are shipped with carbon-fiber filled PTFE piston
seals. Slightly increased abrasion may be observed with
these seals, depending on the application. Consider testing
the permeability of the static mixer regularly at short
intervals.
VF‑pumps The pumps are shipped with UHMW-PE piston seals. Using
tetrahydrofuran, ketones, or ammonium hydroxide as
solvents can damage the seals.
Under harsh conditions, consider replacing the UHMW-PE
piston seals with carbon-fiber filled PTFE piston seals (part
no. 6040.0306). In this case, also install new pistons.
Exchanging the piston seals but keeping the pistons that
were used with the UHMW-PE piston seals may impair the
seal life of the PTFE seals. In addition, note the following:
• Slightly increased abrasion may be observed with the
PTFE seals, depending on the application. Consider
testing the permeability of the static mixer regularly at
short intervals.
• The pump should not be operated with pressures higher
than 70 MPa.
• The pump is no longer biocompatible.
VH‑pumps The pump is shipped with UHMW-PE piston seals. Using
tetrahydrofuran, ketones, or ammonium hydroxide as
solvents can damage the seals.
• For best seal performance, do not recycle or recirculate solvents.
Avoid using methanol from aluminum reservoirs.
• To reduce the growth of algae, consider using amber glassware or
using appropriate additives, for example, formic acid.
• After operation, flush out buffers and solutions that form peroxides.