Parameter
Description
Sample QC - Library DNA
MAPD
The Median of the Absolute values of all Pairwise Differences;
a quality metric that estimates coverage variability between
adjacent amplicons in copy number variant (CNV) analyses. A
MAPD value ≤0.5 generally indicates an acceptable level of
coverage variability in the DNA Library or DNA Control.
Mapped Reads
The total number of bases mapped to target amplicons.
Mean AQ20 Read
Length (bp)
The mean length of sample reads aligned to a reference
sequence that have ≥99% accuracy at each position.
Mean Read Length
(bp)
The mean length of all sample reads.
Uniformity of
Amplicon Coverage
The percentage of reads showing a depth of coverage ≥20% of
the mean base coverage.
Sample QC—RNA
Mapped Reads
The total number of reads mapping to a fusion reference
sequence.
Mean AQ20 Read
Length (bp)
The mean length of called fusion reads that have ≥99%
accuracy at each position.
Mean Read Length
(bp)
The mean length of all sample reads.
Chapter 3
Create and manage assays (manager/administrator)
Create a new assay (manager/administrator)
3
Genexus
™
Integrated Sequencer User Guide
37
