The Genexus
™
Filter captures particulate matter in the liquid waste to prevent
blockage of the waste line over time. The filter needs to be replaced with a new filter
after one year of regular instrument use.
1.
Remove the old filter from the liquid waste disposal port on the instrument deck
by grasping the filter firmly and pulling up. Dispose of the filter as regular waste.
Note:
If an interim filter is installed in the port, use a straight needle probe to
pierce the filter, then lift out the filter using the probe.
2.
Insert a new Genexus
™
Filter into the liquid waste disposal port, then press
firmly to seat the filter O-ring securely.
1
2
3
5
4
1
Genexus
™
Filter
2
Liquid waste disposal port
3
Used pipette tip disposal port
4
Sample plate station
5
Installed filter
Quantify FFPE DNA with the Qubit
™
Fluorometer
When using the Ion AmpliSeq
™
Direct FFPE DNA Kit, the DNA concentration can be
estimated using a Qubit
™
Fluorometer and the Qubit
™
dsDNA HS Assay Kit
(Cat. No. Q32851). See the Qubit
™
dsDNA HS Assay Kits User Guide
(Pub. No. MAN0002326) for more information.
1.
Set up the required number of 0.5-mL Qubit
™
Assay tubes for standards and
samples. The Qubit
™
dsDNA HS Assay requires 2 standards.
2.
Prepare sufficient Qubit
™
working solution for all samples and standards by
diluting Qubit
™
dsDNA HS Reagent 1:200 in Qubit
™
dsDNA HS Buffer.
3.
Combine 2 µL of the FFPE DNA sample with 198 µL (200-µL final volume) of
working solution, mix well, then incubate for at least 2 minutes.
4.
Prepare each Qubit
™
standard as directed in the user guide.
5.
Measure the concentration of each sample and standard on the Qubit
™
Fluorometer.
6.
(Qubit
™
2.0 Fluorometer only.) Calculate the concentration of the undiluted sample
by multiplying by the dilution factor. Alternatively, use the Calculate Stock
Conc. feature on your instrument.
Replace the
Genexus
™
Filter
Appendix C
Supplemental information
Quantify FFPE DNA with the Qubit
™
Fluorometer
C
Genexus
™
Integrated Sequencer User Guide
135
