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Teledyne ProEM Series, System Manual

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4

ProEM System Manual

Issue 3

Chapter 4:

System Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

4.1

Unpacking the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

4.2

Checking the Equipment and Parts Inventory . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  36

4.3

System Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  36
4.3.1

Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  36

4.3.2

Ventilation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  36

4.3.3

Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

4.3.4

Host Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
4.3.4.1

WinX Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

4.3.4.2

LightField Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  38

4.4

Attaching a Lens to a C-Mount Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  38
4.4.1

Mounting the Lens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  39

4.4.2

Adjusting the C-Mount Adapter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  39

4.5

Mounting the Adjustable C- to Spectroscopy-Mount Adapter  . . . . . . . . . . . . . .  40
4.5.1

Positioning the ProEM:512BK Masks. . . . . . . . . . . . . . . . . . . . . . . . . . . . .  42

4.6

Opening/Closing ProEM:1600 Manual Shutter . . . . . . . . . . . . . . . . . . . . . . . . . . . .  42

4.7

Making the Camera-Circulator Connections for a CoolCUBE

II

. . . . . . . . . . . . . .  43

4.8

Software Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  45
4.8.1

WinX/32 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  45

4.8.2

LightField . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  46

4.9

Entering the Default Camera System Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . .47
4.9.1

WinX/32 Versions 2.5.25 and later. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

4.9.2

LightField . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  49

Chapter 5:

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.1

WinX First Light Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
5.1.1

Imaging Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52

5.1.2

Spectroscopy Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  56

5.2

LightField First Light Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
5.2.1

Imaging Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

5.2.2

Spectroscopy Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  65

5.3

Exposure and Signal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  70
5.3.1

Exposure Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  70

5.3.2

Avalanche Gain {EM Gain}. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  70

5.3.3

EM Gain Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

5.3.4

CCD Temperature  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

5.3.5

Dark Charge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

5.3.6

Bias Active Stabilization Engine (BASE). . . . . . . . . . . . . . . . . . . . . . . . . . . .73

5.3.7

Clock Induced Charge (CIC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73

5.3.8

Saturation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74

5.3.9

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75

5.4

Readout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
5.4.1

Dual-Readout Port Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77

5.4.2

Controller Gain {Analog Gain} . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78

5.4.3

Readout Rate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

5.4.4

Region of Interest. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

5.4.5

Binning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81

5.4.6

Exposure – Readout Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  82
5.4.6.1

Frame Transfer Mode (Simultaneous Exposure-Readout)   82

5.4.6.2

Full Frame (Sequential) Mode for Frame-Transfer EMCCD 85

5.4.6.3

Full Frame Readout for Full Frame EMCCD . . . . . . . . . . . . .  86

5.4.7

Readout Time  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87

Summary of Contents for ProEM Series

Page 1: ...www princetoninstruments com ProEM System Manual 4411 0126 Issue 3 September 26 2019 ...

Page 2: ...e Copyright 2009 2019 All Rights Reserved Teledyne Princeton Instruments 3660 Quakerbridge Rd Trenton NJ 08619 TEL 800 874 9789 609 587 9797 2 ProEM System Manual Issue 3 Revision History Issue Date List of Changes Issue 3 September 26 2019 Issue 3 of this document incorporates the following changes Rebranded as Teledyne Princeton Instruments Converted to standard FrameMaker template Issue 2 March...

Page 3: ...and On Chip Multiplication Gain 20 2 2 2 Cooling 20 2 2 2 1 Fan 21 2 2 3 eXcelon 21 2 2 4 Integrated Controller 21 2 2 5 Connectors 22 2 2 5 1 Coolant Ports 23 2 3 Coolant Circulator 23 2 4 Cables 23 2 5 Hoses 24 2 6 Certificate of Performance 25 2 7 Application Software 25 2 8 Accessories 26 2 8 1 CoolCUBEII Circulator 26 2 8 2 C to Spectroscopy Mount Adapter 27 2 8 3 Adjustable C to Spectroscopy...

Page 4: ...ters 47 4 9 1 WinX 32 Versions 2 5 25 and later 47 4 9 2 LightField 49 Chapter 5 Operation 51 5 1 WinX First Light Instructions 52 5 1 1 Imaging Applications 52 5 1 2 Spectroscopy Applications 56 5 2 LightField First Light Instructions 61 5 2 1 Imaging Applications 61 5 2 2 Spectroscopy Applications 65 5 3 Exposure and Signal 70 5 3 1 Exposure Time 70 5 3 2 Avalanche Gain EM Gain 70 5 3 3 EM Gain ...

Page 5: ...tics Option 106 6 5 1 Summary 108 6 6 Custom Modes 108 6 6 1 Custom Chip Custom Sensor 108 6 6 1 1 Software Settings 110 6 6 2 Custom Timing 112 Chapter 7 Tips 115 7 1 Counter the Effects of Aging 115 7 2 Maximize Throughput by Choosing the Right Vacuum Window Coating 115 7 3 Reduce Spectral Readout Time by Using the Custom Chip Timing Custom Sensor Feature 116 7 3 1 Approximation of Spectral Read...

Page 6: ...structions 149 C 3 Adjustable C to Spectroscopy Mount Adapter 7050 0104 for SP2350 SP 2550 150 C 4 Adjustable C to Spectroscopy Mount Adapter 7050 0107 for SP2150 SP 2750 151 C 4 1 Assembly Instructions 151 Appendix D WinSpec 32 LightField Cross Reference 153 D 1 WinSpec 32 to LightField Terminology 153 D 2 LightField to WinSpec 32 155 Warranty and Service 157 Limited Warranty 157 Basic Limited On...

Page 7: ...k Diagram ProEM System 51 Figure 5 2 Typical WinX 32 Controller Camera Tab 53 Figure 5 3 Typical WinX 32 Detector Temperature Dialog 54 Figure 5 4 Typical WinX 32 Experiment Setup Timing Tab 55 Figure 5 5 Typical WinX 32 Controller Camera Tab 57 Figure 5 6 Typical WinX 32 Detector Temperature Dialog 58 Figure 5 7 Typical WinX 32 Experiment Setup Timing Tab 59 Figure 5 8 Typical LightField Availabl...

Page 8: ...tField Custom Timing Pane 112 Figure 6 25 Typical WinX 32 Hardware Setup Controller Camera Tab Vertical Shift 113 Figure 7 1 Vacuum Window Transmission Data 116 Figure 7 2 Typical WinX 32 Easy Bin Dialog 117 Figure 7 3 Typical WinX 32 Readout Time Dialog 2 08 sec 118 Figure 7 4 Typical WinX 32 Hardware Setup Dialog Custom Chip Tab 118 Figure 7 5 Typical WinX 32 Readout Time Dialog 0 88 sec 119 Fig...

Page 9: ...llation Actions 31 Table 5 1 Major Differences Dark Current versus Clock Induced Charge 73 Table 5 2 Typical Controller Gains 78 Table 5 3 Readout Rates 79 Table 6 1 ProEM 512B BK and ProEM 1024B Kinetics versus Spectra Kinetics 106 Table 8 1 List of Recommended Troubleshooting Procedures 123 Table A 1 CCD Specifications by ProEM Model 137 Table A 2 Default Operating Temperature 138 Table A 3 Powe...

Page 10: ...10 ProEM System Manual Issue 3 This page is intentionally blank ...

Page 11: ... collected using an ProEM system This document provides all information necessary to safely install configure and operate the ProEM beginning with the system s initial installation 1 2 Related Documentation Table 1 1 provides a list of related documentation and user manuals that may be useful when working with the ProEM camera system To guarantee up to date information always refer to the current ...

Page 12: ...re for verifying system operation Chapter 6 Advanced Topics This chapter discusses standard timing Trigger Response modes Free Run No Response External Sync Readout Per Trigger and Continuous Cleans Clean Until Trigger Fast and Safe speed modes Logic Level control and the Kinetics mode option Chapter 7 Tips This chapter provides tips regarding CCD ageing maximizing throughput and reducing readout ...

Page 13: ...damage to the equipment being used including but not limited to the possible loss of data WARNING A Warning provides detailed information about actions and or hazards that may result in personal injury or death to individuals operating the equipment WARNING RISK OF ELECTRIC SHOCK The use of this symbol on equipment indicates that one or more nearby items pose an electric shock hazard and should be...

Page 14: ...vided by the equipment may be impaired 2 If the equipment or the wall outlet is damaged the protective grounding could be disconnected Do not use damaged equipment until its safety has been verified by authorized personnel Disconnecting the protective earth terminal inside or outside the apparatus or any tampering with its operation is also prohibited Inspect the supplied power cord If it is not c...

Page 15: ...ly on off state is switched too quickly 4 Use caution when triggering high current switching devices near the system e g an arc lamp The CCD can be permanently damaged by transient voltage spikes If electrically noisy devices are present an isolated conditioned power line or dedicated isolation transformer is highly recommended 5 Do not block air vents on the camera Preventing the free flow of air...

Page 16: ...16 ProEM System Manual Issue 3 This page is intentionally blank ...

Page 17: ...nd coolant hoses Figure 2 1 shows those items that are typically included as part of a standard ProEM Camera system Figure 2 1 Typical ProEM System Components 2 1 1 Optional System Components Optional items include the WinX 32 application software Scientific Imaging ToolKit SITK for LabVIEW a C mount to spectroscopy mount adapter and the Teledyne Princeton Instruments CoolCUBEII coolant circulator...

Page 18: ...e not only for applications that demand the highest available sensitivity but also for those requiring a combination of high quantum efficiency and wide dynamic range Deep thermoelectric cooling and state of the art electronics are employed to help suppress system noise The cameras can be operated at 10 MHz for high speed imaging or more slowly for high precision photometry Supravideo frame rates ...

Page 19: ...try applications and advanced features such as solid baseline stability and linear EM gain control The ProEM 512BK is cooled to below 90 C using either air or liquid or a combination of both ProEM 1024B The ProEM 1024B incorporates a larger 1024 x 1024 13 m pixels frame transfer CCD It provides a large field of view with 13 3 mm x 13 3 mm imaging area and features a high speed EM mode to capture f...

Page 20: ...lent choices for experiments where fast framing and low light sensitivity are required NOTE As the on chip multiplication introduces additional noise it is recommended that the multiplication be used only as required For more information refer to the On Chip Multiplication Gain technical note This technical note can be accessed by going to the Teledyne Princeton Instruments web site www princetoni...

Page 21: ...er if vibration would reduce image quality and the ProEM is also being cooled via a coolant circulator the fan can be turned Off For the fan to function properly free circulation must be maintained between the sides of the camera and the laboratory atmosphere as described above 2 2 3 eXcelon eXcelon is a CCD EMCCD sensor technology jointly developed by Teledyne Princeton Instruments Teledyne e2v a...

Page 22: ...ransmission The cable can extend the distance between camera and the host computer by more than 50 m Shutter LEMO connector provides the shutter drive pulses for driving a Teledyne Princeton Instruments supplied 25 mm external shutter Camera power must be off before connecting to or disconnecting from this connector NOTE When there is an installed internal shutter this connector cannot drive an ex...

Page 23: ...mperature 23 C water and ethylene glycol at 1 liter per minute or the Teledyne Princeton Instruments CoolCUBEII Contact the factory for recommendations Refer to Contact Information on page 160 for complete information 2 4 Cables Table 2 2 describes the cables included with a standard ProEM Camera System LOGIC OUT 0 to 3 3V programmable logic level output TTL compatible The output of this connector...

Page 24: ...mera System Cables Sheet 2 of 2 Cable Part Number Description Purpose Length 4411 0126_0006 4411 0126_0007 Table 2 3 Standard ProEM Camera System Hoses Hose Description Purpose Length Coolant Hoses Two 10 mm 3 8 inch ID hoses are supplied with every ProEM system Quick disconnects that mate to the ProEM s coolant ports have been installed on one end of each hose Refer to the coolant circulator s sp...

Page 25: ...f the detection system into larger experiments or instruments an easy straightforward endeavor PVCam The standard 32 bit software interface for cooled CCD cameras from Teledyne Princeton Instruments It is a library of functions that can be used to control and acquire data from the camera when a custom application is being written For example in the case of Windows PVCam is a dynamic link library D...

Page 26: ...y software packages Please check with the providers of the packages for compatibility and support information 2 8 Accessories Teledyne Princeton Instruments offers a number of optional accessories that are compatible with ProEM For complete ordering information contact Teledyne Princeton Instruments Refer to Contact Information on page 160 for complete information 2 8 1 CoolCUBEII Circulator 4411 ...

Page 27: ... Spectroscopy Mount Adapter 2 8 3 Adjustable C to Spectroscopy Mount Adapter 4411 0126_0010 A C mount to Spectroscopy mount adapter can be ordered separately 4411 0126_0011 An adjustable C to Spectroscopy mount adapter can be ordered separately ...

Page 28: ... damage may have occurred in shipment Retain all original packing materials so that the ProEM system can be easily and safely packaged and shipped to another location or returned for service if necessary If assistance is required at any time contact Teledyne Princeton Instruments Customer Support Refer to Contact Information on page 160 for complete information 2 9 1 Verify Equipment and Parts Inv...

Page 29: ...indow arise due to the accumulation of atmospheric dust we advise that the drag wipe technique be used Before starting the procedure run the camera and disable the shutter open to get access to the window Then dip a clean cellulose lens tissue into clean anhydrous methanol and drag the dampened tissue over the optical surface to be cleaned Do not allow any other material to touch the optical surfa...

Page 30: ...30 ProEM System Manual Issue 3 This page is intentionally blank ...

Page 31: ...e host computer install it Refer to the manufacturer s instructions 5 If the application software is not already installed in the host computer install it Section 4 8 Software Installation on page 45 and software manual 6 Depending on application attach lens to the camera or mount the camera to a spectrometer Section 4 4 Attaching a Lens to a C Mount Adapter on page 38 Appendix C Spectrograph Adap...

Page 32: ...mperature lock wait an additional 20 minutes and then begin acquiring data in focus mode Acquiring Data on page 55 or Acquiring Data on page 63 14 Adjust the focus for the best image or spectral lines If using WinSpec 32 you may want to use the Focus Helper function for spectroscopy applications If you are using LightField you may want to use the Align Spectrometer function Focusing on page 59 Foc...

Page 33: ...nstallation Overview 33 Figure 3 2 Block Diagram Typical Spectroscopy Experiment with Air Cooled ProEM Figure 3 3 Block Diagram Typical Imaging Experiment with Air Liquid Cooled ProEM 4411 0126_0013 4411 0126_0014 ...

Page 34: ...34 ProEM System Manual Issue 3 Figure 3 4 Block Diagram Typical Spectroscopy Experiment with Air Liquid Cooled ProEM 4411 0126_0015 ...

Page 35: ... Instruments Customer Support Refer to Contact Information on page 160 for complete information Hardware installation consists of Installing a dedicated GigE interface card Attaching a lens to a C mount on the camera or to a C to F mount adapter Mounting the camera to a spectrograph for spectroscopy applications Software installation depends on the application software you will be using to run the...

Page 36: ...th ProEM compatible quick disconnects Application Software WinView 32 or WinSpec32 Ver 2 5 25 or later CD ROM optional LightField CD ROM optional Software User Manual provided with application software 4 3 System Requirements 4 3 1 Environmental Requirements Storage temperature 55 C Operating environment temperature 5 C to 30 C the environment temperature at which system specifications can be guar...

Page 37: ...ication of the computer requirements Please contact the factory to determine your specific needs 4 3 4 1 WinX Requirements Windows XP 32 bit with SP3 or later or Vista 32 bit 2 GHz Pentium 4 or greater 1 GB RAM or greater CD ROM drive At least one unused PCI card slot PCI 2 3 compliant 32 bit 33 66 MHz bus Super VGA monitor and graphics card supporting at least 65535 colors with at least 128 MB of...

Page 38: ...f 10 000 RPM recommended Mouse or other pointing device NOTE The above requirements are the minimum for operating a ProEM camera A faster computer with 5 GB or larger memory RAM will greatly enhance the software performance during live mode operations 4 4 Attaching a Lens to a C Mount Adapter CAUTION Overexposure Protection Cameras that are exposed to room light or other continuous light sources w...

Page 39: ...d camera but it should be avoided 4 4 2 Adjusting the C Mount Adapter The ProEM features an adjustable C mount adapter that allows you to change the focal depth Use the hex key supplied with your system or a 0 050 inch hex key to loosen the set screw securing the adapter Using a spanner wrench or equivalent distance between holes is 3 85 inches 97 8 mm rotate the ring to the desired height Tighten...

Page 40: ...ounted to the front of a ProEM camera and is secured to the camera by a threaded insert screwed into the camera s C mount opening Refer to Figure 8 when mounting the adapter to the camera Tools Required 0 050 inch Hex key 3 32 inch hex key Flat screwdriver Spanner wrench 1 17 inch 29 7 mm between 0 094 inch 2 38 mm diameter holes Equipment Teledyne Acton Research Series Spectrograph with light sou...

Page 41: ...ght source powered on and connected to the computer as required start the application software Refer to the appropriate First Light instructions in this manual when focusing and rotationally aligning the camera to the spectrograph optics 6 When you have finished with focusing and rotational alignment secure the sliding tube in place with the spectrograph set screws 7 If they have already been inst...

Page 42: ...ftware to fine tune the masking The pull push knob for the manual shutter allows you to block all light from the camera while you are acquiring a background See Figure 4 3 Figure 4 3 Front View of ProEM 512BK Camera 4 6 Opening Closing ProEM 1600 Manual Shutter ProEM 1600 cameras contain a built in manual shutter that allows you to block all light from the camera while you are acquiring a backgrou...

Page 43: ...Make the coolant connections between the circulator and the camera It does not matter which hose from the circulator is plugged into a coolant port on the camera See Figure 4 5 Figure 4 5 Typical CoolCUBEII Circulator 4 It is recommended that hoses be secured to the camera hose barbs with the clamp supplied NOTE Make sure that there are no kinks in the hoses that impede the coolant flow Lack of su...

Page 44: ... 60 Hz power source 8 Turn the circulator on Make sure there are no leaks or air bubbles in the hoses a If there are no problems proceed to step 9 b If there are leaks or air bubbles turn the circulator off and correct the problem s by securing the hoses or adding more coolant to the reservoir Turn the circulator back on Recheck and if there are no problems proceed to step 9 9 Turn the camera on 1...

Page 45: ...the installation wizard prompts 2 On the Select Installation Type dialog select Typical to install the required drivers and the most commonly installed program files Custom to choose among the available program files or do not want to install the drivers Complete to install all application features See Figure 4 7 Figure 4 7 Typical WinX 32 Setup Dialog Select Installation Type 3 Make sure the came...

Page 46: ...or Windows 7 64 bit Confirm that the GigE adapter card has been installed Verify that your computer is connected to the Internet Internet connection is required for product activation 2 Insert the CD and follow the installation wizard prompts See Figure 4 8 Figure 4 8 Typical LightField Installation Wizard Dialog 3 After the installation finishes reboot the computer 4 Connect the ProEM system comp...

Page 47: ... Welcome dialog leave the check box unselected and click on Next See Figure 4 9 Figure 4 9 Typical WinX 32 Camera Detection Wizard Welcome Dialog 4 Follow the instructions on the dialogs to perform the initial hardware setup this wizard enters default parameters on the Hardware Setup dialog tabs and gives you an opportunity to acquire a single test image to confirm the system is working Note that ...

Page 48: ...mera Tab NOTE For a step by step procedure on basic system operation Imaging and Spectroscopy refer to the appropriate First Light sections Section 5 1 1 Imaging Applications on page 52 Section 5 1 2 Spectroscopy Applications on page 56 4411 0126_0025 SELECT FRAME TRANSFER ...

Page 49: ...riment Devices area the appropriate expanders will be loaded into the Experiment Settings stack on the lefthand side of the window Figure 4 11 Typical LightField Experiment Workspace 5 Because this is a new experiment the default settings will automatically be entered for the experiment device s These settings will allow you to begin previewing Run button or acquiring Acquire button data NOTE For ...

Page 50: ...50 ProEM System Manual Issue 3 This page is intentionally blank ...

Page 51: ...ransferred to the host computer Upon data transfer the data are displayed and or stored via the application software This sequence is illustrated by the block diagram shown in Figure 5 1 Whether or not the data are displayed and or stored depends on the data collection operation that has been selected in the application software Figure 5 1 Functional Block Diagram ProEM System In WinX 32 and Light...

Page 52: ...e exposure readout and digitization factors and making adjustments to software settings you can maximize signal to noise ratio Refer to Chapter 6 Advanced Topics on page 89 for information about synchronizing data acquisition with external devices 5 1 WinX First Light Instructions 5 1 1 Imaging Applications This section provides step by step instructions for acquiring an imaging measurement in Win...

Page 53: ...while performing this procedure Set the software parameters as follows Controller Camera tab Setup Hardware These parameters should be set automatically to the proper values for your system Controller type This information is read from the camera Camera type This information is read from the camera Shutter type Custom System dependent Readout mode Available modes are read from the camera Select Fr...

Page 54: ...be stable to within 0 05 C Figure 5 3 shows the default temperatures for the ProEM 512B and 1024B Figure 5 3 Typical WinX 32 Detector Temperature Dialog NOTE The Detector Temperature dialog will not display temperature information while you are acquiring data Experiment Setup Main Tab Acquisition Experiment Setup Exposure Time 50 ms Accumulations Number of Images 1 Experiment Setup ROI Tab Acquisi...

Page 55: ...y clicking the 5 95 button at the bottom left corner of the data window and adjust the lens aperture until a suitable setting is found Once you ve determined that the image is present select a lower setting for better contrast Check the brightest regions of the image to determine if the A D converter is at full scale A 16 bit A D is at full scale when the brightest parts of the image reach an inte...

Page 56: ...d fluorescent overhead lamps have good calibration lines as well If there are no line sources available it is possible to use a broadband source such as tungsten for the alignment If this is the case use a wavelength setting of 0 0 nm for alignment purposes CAUTION Overexposure Protection Cameras that are exposed to room light or other continuous light sources will quickly become saturated If the ...

Page 57: ...while performing this procedure Set the software parameters as follows Controller Camera Tab Setup Hardware These parameters should be set automatically to the proper values for your system Controller type This information is read from the camera Camera type This information is read from the camera Shutter type Custom System dependent Readout mode Available modes are read from the camera Select Fr...

Page 58: ...t indicate a system malfunction Once lock is established the temperature will be stable to within 0 05 C See Figure 5 6 Figure 5 6 Typical WinX 32 Detector Temperature Dialog NOTE The Detector Temperature dialog will not display temperature information while you are acquiring data Experiment Setup Main Tab Acquisition Experiment Setup Exposure Time 50 ms Accumulations Number of Images 1 Experiment...

Page 59: ... entrance slit of the spectrograph Any light source with line output can be used Standard fluorescent overhead lamps have good calibration lines as well If there are no line sources available it is possible to use a broadband source such as tungsten for the alignment If this is the case use a wavelength setting of 0 0 nm for alignment purposes 2 With the spectrograph properly connected to the came...

Page 60: ... as required to achieve optimum focus Short focal length spectrographs There is generally a focusing mechanism on the spectrograph itself which when adjusted will move the optics as required to achieve proper focus No focusing adjustment If there is no focusing adjustment either provided by the spectrograph or by the mounting hardware then the only recourse will be to adjust the spectrograph s foc...

Page 61: ...he previous sections of this chapter You are familiar with the application software The system is being operated in imaging mode The target is a sharp image text or a drawing that can be used to verify that the camera is seeing and can be used to maximize focus Getting Started 1 Mount a test target in front of the camera 2 Power ON the camera the power switch is on the back of the power supply 3 T...

Page 62: ... Manual Issue 3 Figure 5 8 Typical LightField Available Devices Area 2 Drag the icon into the Experiment Devices area See Figure 5 9 Figure 5 9 Typical LightField Experiment Devices Area 4411 0126_0030 4411 0126_0031 ...

Page 63: ...reached Clicking on the icon opens the Sensor expander which is where the set temperature can be changed Orientation is displayed because the default readout port is Electron Multiplied Clicking on the icon pops a panel that describes how the image orientation was corrected Acquiring Data 1 Click on the View tab just above Experiment Devices to change to the display area See Figure 5 10 Figure 5 1...

Page 64: ... works best Check the brightest regions of the image to determine if the A D converter is at full scale A 16 bit A D is at full scale when the brightest parts of the image reach an intensity of 65535 Adjust the aperture to where it is just slightly smaller higher f stop than the setting where maximum brightness on any part of the image occurs Set the focus adjustment of the lens for maximum sharpn...

Page 65: ...ible to use a broadband source such as tungsten for the alignment If this is the case use a wavelength setting of 0 0 nm for alignment purposes CAUTION Overexposure Protection Cameras that are exposed to room light or other continuous light sources will quickly become saturated If the camera is mounted to a spectrograph close the entrance slit of the spectrograph to reduce the incident light Assum...

Page 66: ...anual Issue 3 Figure 5 12 Typical LightField Available Devices Area 8 Drag the icons into the Experiment Devices area See Figure 5 13 Figure 5 13 Typical LightField Experiment Devices Area 4411 0126_0034 4411 0126_0035 ...

Page 67: ...ve the camera in and out of focus and adjust for optimum while watching a live display on the monitor followed by rotating the camera and again adjusting for optimum The following procedure which describes the focusing operation with a Teledyne Acton Research SP 2356 spectrograph can be easily adapted to other spectrographs The following procedure assumes that the camera and spectrograph have alre...

Page 68: ...If necessary adjust the Exposure Time to maintain optimum near full scale signal intensity 5 Make sure that the spectroscopy mount adapter moves freely at the spectrograph 6 Select Align Spectrometer from the Experiment Options menu Review the displayed information and then click on the Begin button Typically this feature creates three 1 row high ROIs one near the top of the array one in the middl...

Page 69: ...oper focus No focusing adjustment If there is no focusing adjustment either provided by the spectrograph or by the mounting hardware then the only recourse will be to adjust the spectrograph s focusing mirror 8 Next adjust the rotation Click on the peak you want to monitor during the rotational alignment This positions the large cursor to provide a vertical reference line across all of the ROIs 9 ...

Page 70: ... exposure time and subsequent exposures in a sequence will be exposed for the frame readout time Full Frame In this mode the effective exposure time is the set exposure time For complete information about these modes refer to Section 5 4 6 Exposure Readout Modes on page 82 5 3 2 Avalanche Gain EM Gain As explained previously the ProEM uses a unique EMCCD capable of multiplying the charge electrons...

Page 71: ...ng function of the amount of charge that flows through the multiplication register users who consistently operate the camera at high gain at high light levels may need to recalibrate EM gain more frequently than those who are looking at lower light levels at lower gain To compensate for aging each ProEM contains a built in shutter and light source that allow you to perform an on demand EM Gain Cal...

Page 72: ... results you can turn off the fan operation while making sure that the coolant is circulating through the camera to maintain the CCD cooling temperature If the fan is turned off and there is no coolant circulating through the camera the built in thermo protection switch may shut the camera down to prevent thermal damage In the event that this occurs wait about ten minutes correct the situation tha...

Page 73: ...settings and the bias value remains stable over extended sequences Since active bias stabilization is ON by default no user input is required 5 3 7 Clock Induced Charge CIC Clock induced charge CIC is a noise source that must be taken into account when operating EMCCDs at single photon levels As charge is shifted from pixel to pixel during readout a random electron may be generated in the pixel pu...

Page 74: ...d longer exposure times and therefore longer signal accumulation in the CCD will improve the S N ratio approximately linearly with the length of exposure time There is however a maximum time limit for on chip accumulation determined by either the saturation of the CCD by the signal or the loss of dynamic range due to the buildup of dark charge in the pixels CAUTION If you observe a sudden change i...

Page 75: ...l give the best results for most applications Even so it is a good idea to know what these entries mean with regard to cleaning Cleaning Parameters Number of Cleans Number of Clean Cycles value is usually set to one 1 These are additional clean cycles that can be required after a start exposure signal is received and the current clean cycle has finished The maximum value for this entry depends on ...

Page 76: ...p margin inactive parallel strips on a CCD are made up of the dark pixels that come before the active strips on a sensor as they exit to the serial register When these are available i e Pre Dummies Top Margin 0 they serve the purpose of cleaning the serial register before readout of the active strips In LightField if there are no inactive parallel strips i e Top Margin 0 selecting Clean Serial Reg...

Page 77: ...er and the gain i e the number of electrons required to generate an ADU 5 4 1 Dual Readout Port Operation The ProEM 512B BK 1024B 1600 2 and 1600 4 models are configured with software selectable dual readout amplifiers also referred to as ports illustrated in Figure 5 18 The two amplifiers are Port 1 Multiplication Gain Electron Multiplied When this port is selected the EM gain value can be entere...

Page 78: ...ller Gain Analog gain is software selectable and is used to change the relationship between the number of electrons acquired on the CCD and the Analog to Digital Units ADUs or counts generated Selecting the amount of gain is done on the Acquisition Experiment Setup ADC tab Analog to Digital Conversion expander The choices are 1 Low 2 Medium and 3 High Users who measure high level signals may wish ...

Page 79: ...essible after selecting Experiment Setup from the Acquisition menu Easy Bin is a simple way of a defining a single full chip width ROI ROI Setup allows you to create ROIs with greater flexibility in ROI location and width In LightField ROIs are set up via the Region of Interest expander Each ROI is defined in the X and Y direction by a start pixel an end pixel and a group height binning factor Aft...

Page 80: ...er to the horizontal as X or Wavelength depending on the application NOTE This constraint does not apply to LightField Examples These WinX examples include partial frame ROIs with and without binning The terminology is based on the WinX 32 Experiment Setup ROI Setup tab X Start to End 200 pixels no grouping binning Since 200 4 50 this is a valid ROI setup X Start to End 200 pixels and grouping bin...

Page 81: ...d Array Orientation For square format CCDs e g 512 x 512 or 1024 x 1024 you may orient the CCD to achieve binning along either direction of the CCD Binning along columns parallel mode provides maximum scan rate and lowest noise Binning along the rows perpendicular mode minimizes crosstalk and is therefore better for multi spectral applications The drawback to this method is that scanning is slower...

Page 82: ...r so the minimum effective exposure time in this mode is the readout time This mode of operation allows you to continuously image a specimen to obtain better kinetic information about a process In WinX 32 and LightField Frame Transfer is the default mode of operation for cameras with frame transfer CCDs If necessary you can change the readout mode on the Hardware Setup Controller Camera tab Sensor...

Page 83: ...gram d READ OUT SHUTTER IMAGE SHIFT EXPOSE Effective High during the time when the CCD is collecting light EXPOSE Program d High during programmed exposure as entered in the software Can be used for gating the light source SHUTTER High during Shutter open comp Exposure Follows Disabled Opened or Disabled Closed READ OUT High during Vertical Transfer Digitization IMAGE SHIFT High while the image is...

Page 84: ... exposure time is greater than the readout time all frames are precisely exposed for the duration entered into the software and have similar intensities Exposure Time Readout Time Timing information shown is for illustration purpose only Specific readout times vary depending on the model EXPOSE Effective EXPOSE Program d READ OUT SHUTTER IMAGE SHIFT EXPOSE Effective High during the time when the C...

Page 85: ...d for each frame in a sequence Step 1 and Step 3 clearing the CCD and shifting the image are usually very short and do not impact the frame rate In WinX 32 Full Frame mode can be selected from Hardware Setup Controller Camera tab In LightField it is selected on the Sensor expander NOTE Since the software you are using may show the settings differently refer to the software documentation for accura...

Page 86: ... Figure 5 24 the upper left section i e Section 1 represents a full frame EMCCD after exposure but before the beginning of readout The capital letters represent different amounts of charge including both signal and dark charge This section explains readout at full resolution where every pixel is digitized separately In this drawing the charge is being read out of the Low Noise port NOTE The Multip...

Page 87: ...s emptied into this node it is digitized Only after all pixels in the first row are digitized is the second row moved into the shift register The order of shifting in our example is therefore A1 B1 C1 D1 A2 B2 C2 D2 A3 After charge is shifted out of each pixel the remaining charge is zero meaning that the array is immediately ready for the next exposure A subsection of the CCD can be read out at f...

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Page 89: ...tom Timing modes These modes allow you to specify an active sub area of the CCD array and or a faster vertical shift rate for the purpose of increased frame rate pixels outside of the area are not read Custom Chip Custom Sensor mode requires mechanical or optical masking of the array to prevent smearing 6 1 Frame Transfer Full Frame Timing Trigger Response Modes and Shutter Control Shutter Mode Th...

Page 90: ...amera See Figure 6 1 Figure 6 1 Rear of ProEM Camera Things to keep in mind when setting up the External Sync pulse input are Pulse Height 0 to 3 3V logic levels TTL compatible Pulse Width trigger edge frequency The time between trigger edges EXT SYNC Connector Impedance High impedance Trigger Edge Trigger Determined By rising or falling edge must be indicated on the Experiment Setup Timing tab Tr...

Page 91: ...l Sync Readout Per Trigger In this mode each frame in a sequence requires a trigger Each frame is exposed for the length of time entered into the software and is then read out If a trigger arrives during the exposure readout of the previous frame it is ignored See Figure 6 3 Figure 6 3 External Sync Timing Diagram For a sequence of one frame bulb mode and trigger first mode are the same The shaded...

Page 92: ...ed the shutter remains open for the set exposure time then closes If the vertical rows are shifted midway when the External Sync pulse arrives the pulse is saved until the row shifting is completed to prevent the CCD from getting out of step As expected the response latency is on the order of one vertical shift time from 1 30 s depending on the array This latency does not prevent the incoming sign...

Page 93: ...e selected output signal WFT goes HIGH immediately after readout or after preopen if it is active It goes LOW when an active edge or edge depending on your setting occurs and if Continuous Cleans Clean Until Trigger is not enabled the exposure begins If continuous cleans is enabled the camera will check for an active edge at the EXT SYNC connector before entering a continuous clean cycle If none h...

Page 94: ... first trigger in a sequence The subsequent triggers will not run the Continuous Clean Programmed pattern Figure 6 7 illustrates the timing diagram for the following configuration Bulb Trigger Expose During Trigger Pulse Non Overlap Mode Three Exposure Sequence No Preopen No Continuous Cleans Figure 6 7 Timing Diagram Bulb Trigger Expose During Trigger Pulse 6 1 6 Trigger Start Start On Single Tri...

Page 95: ... is that the computer will only display frames when it is not performing other tasks Image display has a lower priority so the image on the screen may lag several images behind A second drawback is that a data overrun may occur if the number of images collected exceeds the amount of allocated RAM or if the computer cannot keep up with the data rate NOTE LightField always uses Fast Mode 6 2 2 Safe ...

Page 96: ...eters Start Safe Mode Start acquisition command sent from computer to camera 1 frame collected as per timing mode Background or flatfield on Background and or flatfield correction performed Frames complete During next acquisition frames are displayed as time permits Stop acquisition command sent from computer to camera Stop No Yes No Yes Cleans performed Computer programs camera with exposure and ...

Page 97: ...Effective Effectively Exposing This level is at a logic high during the effective exposure time This exposure time equals the read out time in frame transfer mode when the exposure time is less than the readout time Otherwise Expose Effective and Expose Program d are identical Expose Program d Exposing This level is at a logic high during the programmed exposure time i e the time set in the softwa...

Page 98: ... is checked the output is at a logic low when the action is occurring 6 4 Kinetics Mode Kinetics refers to a special readout mode in which a portion of the CCD is illuminated while the rest of the active area is used to store a series of frames At the end of the exposure shift sequence the entire CCD is readout to give a series of subframes kinetic frames separated in time In order to support this...

Page 99: ...he cross talk between subframes Typically in imaging applications this is accomplished by placing a knife edge or an optical mask in the collimated beam path Whereas in spectroscopy this is best achieved by limiting the height of the entrance slit of the spectrograph In most of the applications the ability to mask as few rows as possible sets the ultimate limit on the temporal resolution See Figur...

Page 100: ...el applications Window Size 60 Rows furthest from the serial register are illuminated Masked 512 Columns 1048 Rows including reference rows EXPOSURE READ OUT SHUTTER IMAGE SHIFT EXPOSURE High during programmed exposure as entered in the software SHUTTER High during Shutter open comp All Exposures Shift Cycles Follows Disabled Opened or Disabled Closed READ OUT High during Vertical Transfer Digitiz...

Page 101: ...o the shift register divided by the Window Size Integrate signals SHUTTER or Readout signals READ OUT are provided at the LOGIC OUT connector for timing measurements The Shutter Control options are Normal PreOpen Open Before Trigger Disabled Open Always Open and Disabled Closed Always Closed Normal means that the Shutter opens at the beginning of the first exposure shift kinetics cycle and closes ...

Page 102: ...ight to a minimum while multiple trigger mode is used In the case of this mode automatic cleaning of the CCD only occurs until the first trigger arrives Once the series of exposure shift cycles begins cleaning does not occur and at the end of a cycle ambient light will be collected through the window until the next trigger arrives Depending on the time between triggers and the amount of ambient li...

Page 103: ... one row at a time before the arrival of the first trigger This keeps the charge buildup on the CCD to a minimum at the same time minimizing the timing jitter determined by vertical shift time of a single row If desired the number of cleans can be set to zero for the best jitter performance However the camera must be in a dark environment to minimize the background 6 4 5 Configuring a Kinetics Exp...

Page 104: ...ontroller Camera Tab Setup Hardware These parameters should be set automatically to the proper values for your system Controller type This information is read from the camera Camera type This information is read from the camera Shutter type Custom System dependent Readout Mode Available modes are read from the camera Select Kinetics Window Size Enter the number of exposed rows Cleans Tab Setup Har...

Page 105: ...osure Time 100 s Number of Frames 1 Exposures per Frame 1 Region of Interest expander Use the functions on this expander to define the region of interest ROI Clicking on Full Sensor selects the full window without binning Shutter expander Shutter Mode Normal Trigger expander Trigger Response No Response Readout Per Trigger or Shift Per Trigger 6 4 6 Summary Kinetics mode is a powerful feature that...

Page 106: ...mera to acquire the full masked height s worth of spectra regardless of the height of the illuminated rows all while delivering the same temporal resolution as standard kinetics For the fastest possible acquisition this mode requires that an external mask blocks light from all but a window of rows just below the frame transfer area During acquisition the window is exposed and then the rows are shi...

Page 107: ...0 Rows Exposed Frame transfer Area 528 Rows Sensor Area 410 Rows Low Noise For standard high dynamic range applications Multiplication Gain For high speed low light level applications Sensor Area 2 Rows Masked Area 98 Rows 4411 0126_0063 Frame Transfer Mask External Mask Exposed Sensor Area 20 Rows 4411 0126_0064 ...

Page 108: ...ions Custom Sensor and Custom Timing are standard sensor dependent in LightField These modes are intended to allow data acquisition at the fastest possible rates for your camera Custom Chip Custom Sensor allows you to reduce the apparent size of the CCD array and Custom Timing allows you to select a faster vertical shift time 6 6 1 Custom Chip Custom Sensor In addition to Binning and ROI which hav...

Page 109: ...2 ms or a frame rate of 122 fps 1 0 0082 Using the Custom Chip Custom Sensor feature the readout time for the same region would drop to 3 1 ms equivalent to a frame rate of 323 fps See Figure 6 21 for a graphic comparison of the ProEM 512B camera s expected frame rates using standard ROI readout and custom chip readout Figure 6 21 Comparison of Standard ROI and Custom Chip Readout Rates External c...

Page 110: ...the factory for guidance before customizing the chip definition In WinX 32 if Custom Chip has been installed selecting Show Custom Chip check box on the Controller Camera tab adds the Custom Chip tab to the Hardware Setup dialog The Custom Chip parameters are shown in Figure 6 22 The default values conform to the physical layout of the CCD array and are optimum for most measurements Figure 6 22 Ty...

Page 111: ...o that only the reduced active area is exposed This will prevent unwanted charge from spilling into the active area or being transferred to the shift register By default if there are no Pre Dummy rows the serial register will be cleared before rows are shifted If the Skip Serial Register Clean box is selected when there are no Pre Dummy rows the register clean out will be skipped and the chip read...

Page 112: ...iming panel you can select from among the listed vertical shift rate choices see Figure 6 24 Vertical shift rate is the time required to shift one row into the serial register The smaller the value the faster charge will be shifted up one row at a time toward the serial register Figure 6 24 Typical LightField Custom Timing Pane In WinX 32 the equivalent function is located on the Camera Controller...

Page 113: ...Chapter 6 Advanced Topics 113 Figure 6 25 Typical WinX 32 Hardware Setup Controller Camera Tab Vertical Shift 4411 0126_0070 ...

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Page 115: ...by incident photons before they reach the EMCCD detection surface Although the design is the best available each uncoated optical surface of the vacuum window can still have 3 5 to 4 transmission loss or a total loss of 7 to 8 For light starved imaging applications this loss can result in a significant reduction of signal to noise ratio Moreover any light reflected inside the system can lead to gl...

Page 116: ...on Instruments WinX or LightField software These custom modes yield much faster spectral rates than those achievable using a region of interest ROI Teledyne Princeton Instruments PVCAM PICam and National Instruments LabVIEW users can utilize Custom Chip and Custom Timing modes as explained in their respective manuals 7 3 1 Approximation of Spectral Readout Time Calculating the precise spectral rat...

Page 117: ...ted in this procedure These values are intended to show the effect of the changes on Readout Time reduction 1 On the Experiment Setup ADC tab select Rate 4 MHz Readout Port Multiplication Gain 2 On the Hardware Setup Controller Camera tab select Vertical Shift 2 s 3 Select Easy Bin from the Acquisition menu Select ALL This will result in Full Vertical Binning See Figure 7 2 Figure 7 2 Typical WinX...

Page 118: ...tup Controller Camera tab Check the Custom Chip box Select the Custom Chip tab Make the following changes Change Active to 20 Change Pre Dummies to 0 Change Post Dummies to 0 See Figure 7 4 Figure 7 4 Typical WinX 32 Hardware Setup Dialog Custom Chip Tab 7 Now go to the Hardware Setup Controller Camera tab 8 Select Easy Bin from the Acquisition menu Select ALL This will bin the 20 rows together 9 ...

Page 119: ...7 5 Typical WinX 32 Readout Time Dialog 0 88 msec 11 To further reduce the readout time open the Hardware Setup Camera Controller tab set shutter compensation time s to 0 and on the Hardware Setup Custom Camera tab select Skip Serial Register Clean See Figure 7 6 Figure 7 6 Typical WinX 32 Hardware Setup Dialog Custom Chip Tab 4411 0126_0075 4411 0126_0076 ...

Page 120: ...increased spectral readout rate and data quality increasing the frames per second tends to decrease data quality NOTES 1 This same procedure can be used to increase the spectral readout rate for a ProEM 1600 4 2 The Readout Times and Frames Per Second FPS that you get may differ from those reported in this procedure These values are intended to show the effect of the changes on Readout Time reduct...

Page 121: ... Count of 1 The Readout Time is 2 918 ms and FPS is 91 6 7 Set the Shutter Closing Delay time to 0 The Readout Time is 2 918 ms and FPS is 343 8 Set Vertical Shift Time to 2 s The Readout Time is 2 086 ms and FPS is 479 9 In the Custom Sensor fly out pane set Active Area Height to 208 rows and change the Top Margin and Bottom Margin to 0 The Readout Time is 2 086 ms and FPS is 479 10 Change the Re...

Page 122: ...122 ProEM System Manual Issue 3 Figure 7 8 LightField Settings 4411 0126_0078 ...

Page 123: ... Suddenly Changes page 125 Camera Not Found page 126 Camera Stops Working page 126 Camera1 or similar name in Camera Name field page 126 Temperature Lock Cannot be Achieved or Maintained page 128 Gradual Deterioration of Cooling Capability page 128 Data Overrun Due to Hardware Conflict message page 129 Device Is Not Found page 130 Device is Occupied page 131 Ethernet Network is Not Accessible page...

Page 124: ...isible by 4 Figure 8 1 shows an invalid ROI the 30 in 30 x 512 x1 is not evenly divisible by 4 Figure 8 1 Acquisition Display and Invalid ROI If the number of horizontal pixels or super pixels if there is binning in the horizontal is not divisible by 4 go to Experiment Setup On the ROI Setup tab change the Region of Interest ROI and Binning parameters for your experiment so that the resulting numb...

Page 125: ...is displayed If there is a filename click on the camera icon The file data will be cleared from the viewer and the live data will then be displayed Figure 8 2 Acquisition Display 8 3 Baseline Signal Suddenly Changes A change in the baseline signal is normal if the temperature gain or speed setting has been changed If this occurs when none of these settings have been changed there may be excessive ...

Page 126: ...to the computer and has power the wizard should be able to locate it and load it into the Camera Detection Wizard Detected Hardware dialog If the camera is found select it click on the Next button and continue the wizard If the camera is still not detected click on Cancel and contact Teledyne Princeton Instruments Customer Support Refer to Contact Information on page 160 for complete information 8...

Page 127: ...ed in the WinX application directory and rename the camera The new name will then be used by the system until the Camera Detection Wizard is run again To change the default Camera Name 1 Close the application program if it is running 2 Run RSConfig exe from the Windows Start Programs Princeton Instruments menu or from the directory where you installed WinView 32 WinSpec 32 or WinXTest 32 3 Edit th...

Page 128: ...ance should not exceed 10 feet 3 m Typically the camera is at table height and the circulator is on the floor The target array temperature is not appropriate for your particular camera and CCD array The camera s internal temperature may be too high such as might occur if the operating environment is particularly warm if you are attempting to operate at a temperature colder than the specified limit...

Page 129: ...ler array for example a 512 x 512 array To change the DMA buffer setting 1 Note the array size on the Setup Hardware Controller CCD tab or the Acquisition Experiment Setup Main tab Full Chip dimensions 2 Open Setup Environment Environment dialog 3 Increase the DMA buffer size to a minimum of 32 MB 64 MB if it is currently 32 MB or 128 MB if it is currently 64 MB click on OK and close the WinX 32 a...

Page 130: ...onnected and powered on If WinX 32 or LightField is not able to detect a camera that is powered on and connected via the GigE interface UDP ports 20200 20202 may need to be opened These ports must be open before WinX 32 or LightField can detect a Teledyne Princeton Instruments GigE camera but they may have been closed as part of your computer security such as an anti virus program or a firewall Co...

Page 131: ...re updated with the Intel Pro 1000 Grabber Adapter Vision High Performance IP Device driver provided by Pleora Technologies Inc If this computer is connected to an Ethernet network via an Intel Pro 1000 card that does not use the Pleora driver the network connection will be broken To restore the driver for the Ethernet card that is used to connect to the network 1 Locate the EbDriverTool exe file ...

Page 132: ...e array for example a 2048 x 2048 array requires a larger setting than that for a smaller array for example a 512 x 512 array Figure 8 9 Typical WinX 32 Program Error Dialog To correct the problem 1 Click on OK 2 Reboot WinView 32 3 Note the array size on the Setup Hardware Controller CCD tab or the Acquisition Experiment Setup Main tab Full Chip dimensions If your camera contains a large array su...

Page 133: ...ion software is running in Focus mode 8 14 Smeared Images ProEM frame transfer CCDs allow simultaneous exposure readout operations refer to Section 5 4 6 Exposure Readout Modes on page 82 for more information However when the exposure time is small compared to the frame transfer time smearing may appear in the images To alleviate this problem Use a longer exposure time or Use the SHUTTER signal fr...

Page 134: ...odify the default IP address for one of the ports To Modify the Default IP Address for a Port 1 Click on the Windows Start button and click on Control Panel See Figure 8 11 Figure 8 11 Typical Windows Start button 2 On the Control Panel click on Device Manager 3 After the Device Manager opens scroll down until PRO 1000 Grabber Devices or similar label is displayed See Figure 8 12 Figure 8 12 Typic...

Page 135: ...s For example replace the 2 in the IP Address 192 168 2 1 with 4 so the IP Address becomes 192 168 4 1 See Figure 8 14 Figure 8 14 Typical Adapter Properties Settings tab 6 Click on OK 7 When the System Settings Change dialog appears click Yes to restart your computer now or click No to restart it later See Figure 8 15 Figure 8 15 Typical System Settings Change Dialog The change will not take plac...

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Page 137: ...ble For broadband applications a choice of MgFl2 or broadband AR coating is available upon request A 2 CCD Arrays Table A 1 CCD Specifications by ProEM Model Sheet 1 of 2 Parameter ProEM 512B ProEM 512BK ProEM 1024B ProEM 1600 2 ProEM 1600 4 CCD e2v CCD97B PI Proprietary e2v CCD201B PI Proprietary PI Proprietary Image Type Monochrome Monochrome Monochrome Monochrome Monochrome Resolution 512 x 512...

Page 138: ...et ports on the side of the camera allow you to connect the camera to an external coolant circulator Two 10 mm 3 8 ID 3 meter 10 ft coolant hoses with the appropriate fittings for the ProEM are supplied with each system Additional ProEM compatible female barbs part number VL2 F10B P are available from Koolance www koolance com Refer to your circulator s documentation for information about circulat...

Page 139: ...allow data acquisition to be synchronized with external events Trigger edge can be positive or negative going as set in software Synchronization and Timing Modes are discussed in Chapter 6 Advanced Topics MCX to BNC adapter cable is supplied with system Logic Out 0 to 3 3 V programmable logic level output TTL compatible The output of this connector can be programmed and can also be inverted via th...

Page 140: ...plication software and manual Scientific Imaging ToolKit SITK for LabVIEW C mount to spectroscopy mount adapter adjustable C to spectroscopy mount adapter and the CoolCUBEII coolant circulator Contact Teledyne Princeton Instruments Customer Service for more information regarding options available for your system Refer to Contact Information on page 160 for complete information A2 TEC Power 1 INT R...

Page 141: ...LE FEMALE THREAD 10 32 UNF 0 313 0 0 690 CCD NOMINAL LOCATION 17 53 3 41 86 7 7 89 200 3 8 02 203 7 INLET OUTLET COOLANT PORTS 3 8 HOSE BARB FITTINGS 0 0 62 15 7 1 73 43 8 0 63 15 9 1 76 44 6 0 0 24 6 0 1 49 37 7 0 50 12 8 1 25 31 9 STATUS LED 2 PIN LEMO MCX CONNECTORS 0 1 13 28 6 1 13 28 6 0 1 15 TRIPOD HOLES ALL 4 SIDES 29 2 0 90 22 9 NOTES 25mm INTERNAL SHUTTER 1 WINDOW MATERIAL FUSED SILICA TH...

Page 142: ...CK DISCONNECT FITTINGS BOTTOM ADJUSTABLE MASK TOP ADJUSTABLE MASK 1 50 38 1 32 UN THREAD SHUTTER KNOB 8X 10 32 UNF MOUNTING HOLES 0 0 333 LOCATION OF ADJUSTABLE MASKS 7 863 199 71 7 996 203 09 3 356 85 24 1 125 28 58 0 1 486 37 74 0 1 725 43 82 1 755 44 58 1 300 33 03 0 625 15 88 STATUS LED MCX CONNECTORS 2 PIN LEMO SLIDERS TO OPERATE EXTERNAL MASKS NOTES INTERNAL MANUAL SHUTTER 1 WINDOW MATERIAL ...

Page 143: ...4 21 2 0 2 90 73 7 MANUAL SHUTTER ACTUATOR 8X 10 32 UNF 0 313 0 0 59 CCD NOMINAL 15 0 LOCATION 1 01 25 7 3 24 82 3 7 73 196 3 7 86 199 6 COOLANT INLET OUTLET QUICK DISCONNECT FITTINGS 0 0 62 15 7 1 72 43 7 1 75 44 5 0 63 16 0 0 1 49 37 7 1 26 31 9 0 51 12 8 MCX CONNECTORS INTERNAL MANUAL SHUTTER PUSH TO OPEN PULL TO CLOSE 1 WINDOW MATERIAL FUSED SILICA THICKNESS 0 125 2 UNIVERSAL TRIPOD ADAPTER SU...

Page 144: ...44 ProEM System Manual Issue 3 Figure B 4 Outline Drawing ProEM Power Supply 0 1 06 4 02 2 16 0 2 15 4 29 0 18 POWER CONNECTOR RED LED GREEN LED LINE CORD INPUT POWER ON OFF SWITCH 0 9 82 4411 0126_0098 ...

Page 145: ...72 7 0 1 30 33 0 3 00 76 2 5 00 127 0 8 00 203 2 0 80 20 3 7 20 182 9 2 45 62 2 0 6 12 155 3 1 83 46 4 3 89 98 9 0 0 68 17 3 1 77 45 0 3 69 93 7 DO NOT COVER VENTILATION HOLES 0 2 00 50 8 4 97 126 3 0 5 70 144 7 EXHAUST OPENING AC RECEPTACLE NO SPILL QUICK DISCONNECT SHUT OFF FITTINGS REMOVE RESERVOIR CAP TO REPLENISH COOLANT REFER TO OWNER S MANUAL FOR PROPER COOLANT REFILL PROCEDURE POWER ON OFF...

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Page 147: ...and sliding tube NOTE When mounted to a spectrograph the text on the back of the ProEM should be right side up Table C 1 cross references the adapter kits with the page number for the appropriate instruction set Table C 1 ProEM Spectrograph Adapter Kit Cross Reference Spectrograph Adapter Kit Number Information begins on Teledyne Acton Research ProEM with 3 60 3 88 Bolt Circles page 148 Teledyne A...

Page 148: ...the camera and secure them to the camera nose with the screws 7 Rotate the camera and sliding tube assembly as you gently insert it into the spectrograph 8 Secure the sliding tube with the two setscrews NOTE Adapter parts are machined to provide a tight fit If you need to remove the sliding tube from the spectrograph first loosen the two setscrews that secure it and then rotate the tube as you pul...

Page 149: ...pter plate 6 Gently rotate the camera and sliding tube assembly as you insert it into the spectrograph 7 Secure the sliding tube with the two setscrews NOTE Adapter parts are machined to provide a tight fit If you need to remove the sliding tube from the spectrograph first loosen the two setscrews that secure it and then rotate the tube as you pull it out If you have removed the sliding tube from ...

Page 150: ...it into the spectrograph NOTE 5 Secure the sliding tube with the two spectrograph setscrews one coming down from Adapter parts are machined to provide a tight fit If you need to remove the sliding tube from the spectrograph first loosen the two setscrews that secure it and then rotate the tube as you pull it out If you have removed the sliding tube from the spectrograph rotate the sliding tube as ...

Page 151: ...spectrograph 5 Secure the sliding tube with the two spectrograph setscrews one coming down from the top and the other from the side NOTE Adapter parts are machined to provide a tight fit If you need to remove the sliding tube from the spectrograph first loosen the two setscrews that secure it and then rotate the tube as you pull it out If you have removed the sliding tube from the spectrograph rot...

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Page 153: ... Width ADC Rate Speed ADC Resolution Bit Depth Continuous Cleans Clean Until Trigger Controller Gain Analog Gain Custom Chip Custom Sensor Custom Timing Custom Timing Disabled Closed Shutter Always Closed Shutter Disabled Open Shutter Always Open Shutter Dual Trigger Mode Shift Per Trigger Easy Bin Sensor Readout Region expander functions Edge Trigger Trigger Determined By External Sync Readout Pe...

Page 154: ...ight Margin Pre Dummy Rows Parallel to Shift Register Active Area Top Margin Pre Dummy Shift Register Columns Active Area Left Margin PreOpen Shutter Open Before Trigger Shutter Readout Port Quality Shutter Close Compensation Time Closing Delay Shutter Control Shutter Mode Shutter Open Compensation Time Opening Delay Single Trigger Mode DIF Readout Per Trigger Skip Serial Register Clean deselected...

Page 155: ...ys Closed Shutter Disabled Closed Shutter Always Open Shutter Disabled Open Shutter Analog Gain Controller Gain Bit Depth ADC Resolution Clean Cycle Height Number of Strips per Clean Clean Serial Register Skip Serial Register Clean deselected Clean Until Trigger Continuous Cleans Closing Delay Shutter Close Compensation Time Custom Sensor Custom Chip Custom Timing Custom Timing Final Section Count...

Page 156: ...DIF Sensor Readout Region expander functions Easy Bin Shift Per Trigger DIF Dual Trigger Mode DIF Shutter Mode Shutter Control Speed ADC Rate Temperature Setpoint Target Temperature Trigger Determined By Edge Trigger Trigger Response Timing Mode Table D 2 LightField to WinSpec 32 Cross Reference Sheet 2 of 2 LightField Term WinSpec 32 Term ...

Page 157: ...RANTIES EXPRESS OR IMPLIED this product against defects in materials or workmanship for a period of up to one 1 year after shipment During this period Teledyne Princeton Instruments will repair or replace at its sole option any defective parts without charge to you You must deliver the entire product to the Teledyne Princeton Instruments factory or at our option a factory authorized service center...

Page 158: ...e burn damage which carry NO WARRANTIES EXPRESSED OR IMPLIED all image intensifier products for a period of one 1 year after shipment Refer to additional Limited One 1 year Warranty terms and conditions above which apply to this warranty Responsibility for shipping charges is as described above under our Basic Limited One 1 Year Warranty X Ray Detector Limited One Year Warranty Teledyne Princeton ...

Page 159: ...tion an authorized service center 4 Before products or parts can be returned for service you must contact the Teledyne Princeton Instruments factory and receive a return authorization number RMA Products or parts returned for service without a return authorization evidenced by an RMA will be sent back freight collect 5 These warranties are effective only if purchased from the Teledyne Princeton In...

Page 160: ...nd countries do not allow limitations on how long an implied warranty lasts when an action may be brought or the exclusion or limitation of incidental or consequential damages so the above provisions may not apply to you 12 When contacting us for technical support or service assistance please refer to the Teledyne Princeton Instruments factory of purchase contact your authorized Teledyne Princeton...

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Page 162: ...A 1 877 474 2286 France 33 1 60 86 03 65 Germany 49 0 89 660 7793 UK Ireland 44 0 1628 472 346 Singapore 65 6408 6240 China 86 10 659 16460 Japan 81 3 5639 2741 Copyright 2019 Teledyne Princeton Instruments All Rights Reserved 02 2019 ...

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