Teledyne ProEM Series, System Manual

Page: 78 / 162

78 ProEM System Manual Issue 3
• Port #2: Low Noise (Normal CCD)
When the camera is using this amplifier, electrons (signal) generated in pixels
are clocked through the standard serial register. The amplifier is designed to
take advantage of the dynamic range of the CCD and is most useful when the
frame rate is not critical. (e.g., bright field, fixed cell fluorescence, etc.)
NOTE:
An image read out of the Low Noise port is the mirror image
of the same image read out of the Multiplication Gain
{Electron Multiplied} port. Unless the application software
automatically corrects the orientation when the selected
port changes, you may need to specify a horizontal-flip of
the image.
If your camera is configured with two readout amplifiers (ports), the software
automatically allows port selection.
NOTE:
Because the user interface may be different, refer to your
software manual for specific information about readout
amplifier/port selection.
5.4.2 Controller Gain {Analog Gain}
Controller Gain {Analog gain} is software-selectable and is used to change the
relationship between the number of electrons acquired on the CCD and the
Analog-to-Digital Units (ADUs or counts) generated.
Selecting the amount of gain is done on the Acquisition
►
Experiment Setup…
►
ADC
tab {Analog to Digital Conversion expander.} The choices are 1 {Low}, 2 {Medium}, and 3
{High}. Users who measure high-level signals may wish to select Low to allow
digitization of larger signals. Medium is suitable for experiments within the mid-level
intensity range. Users who consistently measure low-level signals may wish to select
High, which requires fewer electrons to generate an ADU and reduces some sources of
noise.
The Certificate of Performance supplied with the camera lists the measured gain values
at all settings. Typical values are provided in
Table 5-2 .
Table 5-2: Typical Controller Gains
Readout
Amplifier (Port)
Readout Rates
Typical Gain for
512B(e- /ADU)
Typical Gain for
1024B (e- /ADU)
Multiplication
Gain {Electron
Multiplied} 5 MHz
10 MHz
#1: 12e
-
/ADU
#2: 6e
-
/ADU
#3: 3e
-
/ADU
#1: 12e
-
/ADU
#2: 6e
-
/ADU
#3: 3e
-
/ADU
Low Noise 100 kHz
1 MHz
5 MHz
#1: 3.2e
-
/ADU
#2: 1.6e
-
/ADU
#3: 0.8e
-
/ADU
#1: 3e
-
/ADU
#2: 1.5e
-
/ADU
#3: 0.7e
-
/ADU