Teledyne ProEM Series, System Manual

Page: 77 / 162

Chapter 5 Operation 77
The ProEM:1600
(2)
and ProEM:1600
(4)
models use full frame CCDs with 1600 x 200 and
1600 x 400 active pixels, respectively.
NOTE:
Typically there are additional rows and columns for internal
reference.
In standard frame transfer mode, the sensor area is exposed for certain time and the
acquired image data are then transferred to frame transfer area before reading the data
out via the multiplication gain register or standard serial register.
In standard full frame transfer mode, the sensor area is exposed for certain time and the
acquired image data are then read out via the selected register.
Region of Interest (ROI) and/or binning can be used to improve the time resolution,
limited to a millisecond regime.
For additional information about the CCD exposure-readout operations, refer to
Section 5.4.6, Exposure – Readout Modes , on page 82.
WinX/32 and LightField allow you to specify the type of readout (i.e., full frame or
binned,) the output amplifier, and the gain (i.e., the number of electrons required to
generate an ADU.)
5.4.1 Dual-Readout Port Operation
The ProEM:512B/BK, 1024B, 1600
(2)
, and 1600
(4)
models are configured with
software-selectable dual-readout amplifiers, also referred to as ports, illustrated in
Figure 5-18 .
The two amplifiers are:
• Port #1: Multiplication Gain {Electron Multiplied}
When this port is selected, the EM gain value can be entered on the Experiment
Setup
►
Main tab {Analog to Digital Conversion expander.} Since the
multiplication gain can be used to overcome the read noise of the fast amplifier,
this mode is most useful in applications requiring low-light sensitivity at high
frame rates (e.g., Single molecule fluorescence, ion imaging, etc.). Only 25x –
100x EM gain is required to overcome the read noise of the camera. Using
excessive EM gain will not improve the overall signal to noise ratio (SNR).
NOTE:
An image read out of the Multiplication Gain {Electron
Multiplied} port is the mirror image of the same image read
out of the Low Noise port. Unless the application software
automatically corrects the orientation when the selected
port changes, you may need to specify a horizontal-flip of
the image.