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Chapter 5: Operation
Once the ProEM camera has been installed as explained in the preceding chapters,
operation of the camera is straightforward. In most applications you simply establish
optimum performance using the Focus {Preview} mode (in WinX, for example), set the
target camera temperature, wait until the temperature has stabilized, and then do
actual data acquisition in the Acquire mode. Additional considerations regarding
experiment setup and equipment configuration are addressed in the software manual.
During data acquisition, the CCD array is exposed to a source and charge accumulates
in the pixels. After the defined exposure time, the accumulated signal is readout of the
array, digitized, and then transferred to the host computer. Upon data transfer, the data
are displayed and/or stored via the application software. This sequence is illustrated by
the block diagram shown in
Whether or not the data are displayed and/or stored depends on the data collection
operation that has been selected in the application software.
Figure 5-1: Functional Block Diagram: ProEM System
In WinX/32 and LightField, the data collection operations use the Experiment Setup
parameters to establish the exposure time (the period when signal of interest is allowed
to accumulate on the CCD). Focus {Preview} is more likely to be used in setting up the
system (refer to the First Light discussions) and Acquire is then used for the collection
and storage of data.
Incoming Photons
Camera
EMCCD
Preamp Electronics
Analog to Digital
Converter
Digital Processor
GigE Interface
Computer
GigE Interface
Display
Storage
441
1-
0
12
6
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0
0
2
7
Summary of Contents for ProEM Series
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