Section G – Dissolution Testing
CD14 Tester & CD AutoPlus Autosampler User Guide—75-108-800 Rev. B—31 May 2021
86 of 130
EAR99 technology subject to restrictions on cover page.
Automated (Midpoint): In Midpoint mode the instrument
calculates how long it will take to load the sample, and
then it adjusts operations such that the midpoint of the
complete sampling cycle occurs at the specified
sample time point. In this mode the syringe will begin to
load the sample just prior to the programmed time point
such that the midpoint of the cycle will fall within a few
seconds of the specified sampling time. For example, if
the sample collect volume is 8 ml, and Midpoint mode
is selected, then at the programmed sample time point
about 4 ml of sample will already have been loaded
into the syringe. If the sample (collect volume) is larger
than 10 ml, the calculated duration of the complete
sampling cycle will include the time needed to dispense
the full syringe before the rest of the sample is loaded.
Rack Type: The type of rack used for sample collection during
the test. Options are 16 x 100 or 12 x 32.
Rinse Volume: The amount of volume pumped before
collection is done. The range is 0 – 99.9 mL. This volume
should be large enough to ensure that old material in the
sampling lines is rinsed to ensure the sample collected is
representative of what is in the vessels of the tester. This
parameter applies only in automated sampling mode. The
default value is 7 mL.
Collect Volume: The volume collected into test tubes or vials
during a sampling cycle. The range is 0 – 99.9 mL. The
maximum for 16 x 100 test tubes is recommended to be no
more than 15 mL; for 12 x 32 vials, no more than 1.5 mL. This
parameter applies only in automated sampling mode.
NOTE: If the collect volume is larger than the capacity of the
test tube or the vial, the sample will be collected into multiple
rows of vials or test tubes.
Vessel Offset Time: In manual mode, the dosage is dropped
and the vessels are each sampled sequentially, by this fixed
interval. The vessel offset time should be long enough to allow
for the user to sample a vessel and get ready for the next one.
After the test length elapses, the stirring time is extended by
the vessel offset time times the number of vessels (6 or 12).
Vessel offset time is not used during automated sampling as
the dosage is dropped and the samples are taken
simultaneously.
