Section I – CD AutoPlus Maintenance
CD14 Tester & CD AutoPlus Autosampler User Guide—75-108-800 Rev. B—31 May 2021
108 of 130
EAR99 technology subject to restrictions on cover page.
Using the equation
1.
Obtain the average of the blanks (A
0%
).
2.
Obtain the average of the last two absorbing solution
samples (A
100%
).
3.
Subtract the value from step 1 from the value of step
2. (step 2 minus step 1)
(A
100%
- A
0%
)
4.
Multiply the value from step 3 by 0.99 (99%)
(A
100%
- A
0%
) * 0.99
5.
Divide the value obtained in step 4 by 1.00 (100%).
((A
100%
- A
0%
) * .99) / (1.00)
6.
Add the values in step 5 and step 1 for the final value.
Carryover
The carryover evaluation tests the rinse volume to ensure it is
true for all the lines in the system. This test is performed on all
lines used, but only on source A.
Materials required
1.
One UV/Vis spectrophotometer
2.
One absorbing solution with a UV absorbance of
approximately 1.00 AU
a. Caffeine solutions or prednisone solutions are
suitable choices.
b. An absorbance of 0.90 to 1.10 AU is
recommended.
3.
DI or higher quality water
4.
Test tubes
a. Vials are not recommended due to sample size.
b. Recommended sample size is 7 – 10 mL for UV
analysis.
