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BluePippin Operations Manual 460013 Rev F
1-3
1.4 Collection Types
The BluePippin can be programmed to size select either
targeted fractions or “High-Pass” collections. The fragment
size distributions are described below:
1. Minimum Size Distribution (“Tight” setting). The
cassette lane will collect the narrowest distribution of
fragments that is achievable given the resolution of
the agarose gel concentration within the cassette.
The distribution is Gaussian, and its range is typically
+/- 16% (CV 8%) of the median fragment size.
2. Range Size Distribution (“Range” setting). The
cassette lane will collect a distribution of fragments
between a starting base pair value and an ending
base pair value. The actual start and end fragment
sizes are typically /- 10% of the base pair
values input into the software.
3. High-
Pass Size Distribution (uses “Range*”
setting). The cassette lane will collect all
fragments above a base pair threshold. The
method increases the average fragment length of
the collection depending on threshold value and
input DNA distribution profile. High-Pass protocols
require and external marker to be run in one lane.
*
Although the cassette continues to collect until the end of the run,
an end base pair value will auto-fill in the software protocol field.
This value should be disregarded, and should not be changed by
users.
Range
Tight
High Pass