978-922-1832
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www.sagescience.com
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BluePippin Operations Manual 460013 Rev F
1-1
1
Introduction
Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an
automated preparative gel electrophoresis system.
We urge you to read this manual to familiarize yourself with the system’s capabilities and
precautions.
1.1 System Overview
There are three components to the BluePippin system:
•
Instrument
–
The instrument includes epifluorescence detectors, an
electrophoresis power supply, an electrode array, and a Linux-based single-
board computer. The system computer is accessed by an external LCD
monitor, mouse, and keyboard. The electrode array is located in the top of the
sliding instrument cover and is not user-accessible.
•
Software
– System software allows the user to enter parameters that define
the DNA fragment size ranges to be collected. The software also logs
fluorescence and electrical current data which may be analyzed in a review
screen.
•
Gel Cassettes
– Pre-cast, disposable agarose cassettes are manufactured by
Sage Science. Cassettes are supplied in kits that contain 10 cassettes and
sufficient reagents to complete all collections. Kits have been developed to
address DNA size range collections at various size ranges. Please refer to
Section 23
for kit descriptions and ordering numbers.
Sliding Lid:
electrodes are
embedded inside
the lid
Nest
Gel Cassette
Epi-fluorescent
detectors
The BluePippin Instrument
(the monitor and keyboard are not shown)