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BluePippin Operations Manual 460013 Rev F
15-1
15
System Validation
Control DNA can be purchased from Sage Science to check the performance of the
BluePippin system. The Control DNA is useful to test, refine, and troubleshoot
BluePippin size fractionation protocols. For comparison to factory tested cassettes, an
extraction protocol and typical Agilent Bioanalyzer results are provided with the kits.
Figure 15.1
illustrates QC size selection protocol for the 2% agarose control kit, and
Figure 15.2
shows a typical result from a 150 bp tight extraction from the kit guide.
BluePippin cassettes and instruments are functionally tested using restriction digests of
genomic DNA from E. coli. For each cassette type, a different restriction digest is used,
chosen so that size distribution of the digested DNA closely matches the useful
fractionation range of the cassette, without any significant peaks or discontinuities.
Following restriction digestion, the control DNA is purified by phenol:chloroform
extraction, dialyzed, and diluted into BluePippin electrophoresis. Each kit contains
sufficient control DNA to fully load four gel cassettes.
Figure 15.1 Control DNA profile (with reference marker) and DNA extraction
protocol for a 2% agarose DNA control kit run.
Figure 15.2 Example of a Bioanalyzer result for the 150 bp target of a Control
DNA run on a 2% Agarose Gel Cassette
Important!
Data are not intended to imply guaranteed results or performance. Control standards
are intended to demonstrate that the BluePippin system is functioning as expected, and that
proper operational technique is being used.