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BluePippin Operations Manual 460013 Rev F
10-4
Important!
Make sure that the pipette tips used for step 4 extend all the way to the bottom of the
elution modules without sealing the elution port opening. If the tips seal the port opening, it will be
extremely difficult, or impossible, to empty and refill the elution module completely. Test tip fit
using the empty rinse cassette supplied with the instrument.
Note: The total volume of the elution module when filled to the top is 65
l. The specified starting
volume of 40
l only partially fills the module.
5.
Seal the elution wells with the adhesive tape strips.
Tape for sealing the elution wells
are supplied with cassette packaging. Long elutions will cause the elution wells to
overflow if not sealed.
Place tape over the elution wells and rub firmly to fix the tape in
position. For best results, rub the tape at the boundary of the well with a smooth hard
object such as the back end of a lab marker pen.
6.
Check the buffer levels in the sample wells.
Sample wells should be completely filled
to the top with buffer. If any wells are under-filled, top them off with additional buffer.
Note:
The total volume of the sample well is approximately 70
l.
10.3 Continuity Test
The continuity test measures the current in each separation and elution channel and
determines whether they are within the expected values for a successful run.
Important! Temperature affects electrical current readings. If cassettes have been refrigerated,
they will fail the current test until the cassette is at least 17
o
C (62
o
F). Should this be the case,
wait until the cassette temperature has equilibrated to room temperature and re-test.
1.
Press “Test”.
With the cassette in the optical nest, c
lose the lid and press “TEST” on
the controller on the Main Tab.