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BluePippin Operations Manual 460013 Rev F
5-1
5
Workflow Summary
Prepare samples
add loading solution/standard mix to samples
max. 5 samples/cassette
Use internal standard
or external marker?
internal standard
HMW protocol?
(>2kb, 0.75% agarose)
Yes
No
Prepare samples
add loading solution to samples
use external marker
max.4 samples/cassette
external marker
Program a Size Selection Protocol
Select “USE INTERNAL STANDARDS”
Program a Size Selection Protocol
Enter reference lane No. for the external marker
Select “APPLY TO ALL LANES”
Calibrate the Optics
Prepare the Cassette
Run the Continuity Test on the Cassette
Load Samples onto the Cassette
Run the BluePippin