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BluePippin Operations Manual 460013 Rev F
14-2
14.3 Improving Product Yield by Selecting a Wider Size Range
The BluePippin can produce very narrow size distributions from sheared genomic DNA.
However, narrower size distributions will necessarily mean that a smaller fraction of the
input DNA will be recovered. Users should broaden their collection ranges if the default
tight settings do not produce enough DNA for their application.
Figure 14.2
shows an example of the effect of size range selection vs. yield.
Figure 14.2
. Increasing yield by widening the size selection range.
Note.
The percent recovery specification is determined by collecting known amounts of
restriction fragments (Section 14.2). Product yield from a sheared DNA sample is influenced by
the fragment distribution and input profile of the sample.