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BluePippin Operations Manual 460013 Rev F
13-1
13
Sample Collection
13.1 Overview
1.
Remove the sealing tape strip that covers the
elution wells.
2.
Samples can be removed from elution modules using a standard 100-200µl pipette.
Important!
Make sure that the pipette tips used for collection extend all the way to the bottom of
the elution modules without sealing the elution port opening. If the tips seal the port opening, it will
be extremely difficult, if not impossible, to completely recover the contents of the elution module.
Test tip fit using the empty rinse cassette supplied with the instrument.
3.
Samples from standard cassettes with sealed elution modules will be recovered in
approximately 40
l of electrophoresis buffer.
4.
When all samples are collected, remove used cassette from instrument and dispose of it
properly.
Important!
Used cassettes should never be allowed to sit in closed instruments for long periods
of time (e.g., more than one day, overnight is acceptable). Under those conditions, humidity from
the cassette reservoirs can accelerate corrosion of the electrode assembly, located in the sliding
cover.
Note.
Electrophoresis buffer is 50 mM Tris, 30 mM TAPS, 0.1 mM EDTA. During elution, EDTA
does not rapidly equilibrate across the ultrafiltration membrane of the elution module, and so the
final concentration of EDTA in eluted samples is elevated to 1-2 mM
Note:
Eluted samples can be used directly for ligation and amplification without buffer exchange.