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BluePippin Operations Manual 460013 Rev F
10-1
10
Preparing a Cassette
10.1 Visually Inspect the Cassette
1.
Remove the cassette from foil packaging.
Foil package is scored at one end to allow
tearing.
2.
Inspect the levels of
buffer in the buffer reservoirs.
Tip the cassette to consolidate
any bubbles in the reservoirs, then hold the cassette in a horizontal position and look at
the reservoirs from the top and bottom edges. Reservoirs should be nearly full of buffer
and roughly equal in volume across the cassette. If the buffer level in any reservoir
appears less than 50% full (compared with its neighbors), the low reservoir should be
refilled with the supplied electrophoresis buffer prior to running. When refilling, a visual
check of buffer levels is sufficient -- a measured volume is not required.
Figure 10.1
. Low buffer levels in cassette buffer chambers
.
3.
Inspect the gel columns.
Look for obvious breakage of the agarose column in each
channel.
Important! If there is obvious breakage, do not use the lane. Remaining lanes can be used
.
buffer volumes are Low
(+) side of cassette
(-) side of cassette