978-922-1832
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www.sagescience.com
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BluePippin Operations Manual 460013 Rev F
iv
10
PREPARING A CASSETTE ........................................................................................... 10-1
10.1 Visually Inspect the Cassette ...................................................................................... 10-1
10.2 Prepare the Cassette for Loading ................................................................................ 10-3
10.3 Continuity Test ............................................................................................................... 10-4
11
LOADING SAMPLES ..................................................................................................... 11-1
12
RUNNING A PROTOCOL .............................................................................................. 12-1
12.1 Overview ......................................................................................................................... 12-1
12.2 Starting a Run................................................................................................................. 12-2
12.3 Monitoring a Run ........................................................................................................... 12-3
12.4 Log Files ......................................................................................................................... 12-5
13
SAMPLE COLLECTION ................................................................................................ 13-1
13.1 Overview ......................................................................................................................... 13-1
14
SAMPLE RECOVERY AND YIELD ................................................................................ 14-1
14.1 Overview ......................................................................................................................... 14-1
14.2 Intrinsic Sample Recovery on the BluePippin ............................................................. 14-1
14.3 Improving Product Yield by Selecting a Wider Size Range ........................................ 14-2
14.4 Improving Product Recovery with the Field Reversal ................................................. 14-3
14.5 HMW DNA: Recommendations to Improve Yield .......................................................... 14-4
15
SYSTEM VALIDATION .................................................................................................. 15-1
16
SYSTEM OPTIONS TAB ............................................................................................... 16-1
17
RUNNING IN MANUAL MODE ...................................................................................... 17-1
18
ANALYZING RUNS - LOG REVIEW TAB ...................................................................... 18-1
19
MANAGING FILES -- FILE MANAGER TAB ................................................................. 19-1
19.1 Overview .......................................................................................................................... 19-1
19.2 File Types ......................................................................................................................... 19-2
19.3 Transferring files ............................................................................................................. 19-3
20
UPGRADING SOFTWARE ............................................................................................ 20-1
20.1
Extracting the Files to a USB flash drive ..................................................................... 20-1
20.2 Upgrading the Pippin Instrument Software .................................................................. 20-3
CONTENTS
(Cont’d)