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BluePippin Operations Manual 460013 Rev F
7-7
7.6 Assigning Reference Markers
– internal standards
1. Markers must be loaded into every lane with the sample (30 ul of sample with 10 ul of
internal standard loading solution).
2.
In the protocol editor, press “USE INTERNAL STANDARDS”. This will auto-fill the “Ref
Lane” fields with reference marker internal to the sample. The software will determine
the timing of elution based on the migration of the DNA marker within its own lane.
3.
Figure 7.2
shows the correct assignation of marker lanes for internal standard runs.
Figure 7.2
. The correct configuration for a protocol using internal standards.
Important!
When using internal standards all samples must be prepared with the BluePippin
internal standard loading solution which is formulated for the cassette type and size range to be
used. The internal standard loading solution contains the markers.
All lanes will reference off of
internally loaded standard