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BluePippin Operations Manual 460013 Rev F
4-5
Unpacking Gel Cassettes
Gel cassettes are shipped in boxes in the following configurations. Ensure boxes are in
the upright position and confirm that the following contents are present:
Dye-free gels with internal standards
(50 samples, size selections 90 bp
– 1.5 kb):
o
10 foil-sealed gel cassettes
(store at R.T.)
o
1 package of adhesive tape for sealing elution wells
o
1 reagent kit for 10 cassettes
(store at 4
o
C)
▪
550
μl DNA internal standards/ loading solution mix
(10
μl/sample)
▪
40 ml of spare running buffer
Dye-free gels with labeled external marker
(40 samples, size selections >2kb):
o
10 foil-sealed gel cassettes
(store at R.T.)
o
1 package of adhesive tape for sealing elution wells
o
1 reagent kit for 10 cassettes
(store at 4
o
C)
▪
440
μl DNA size markers (1 x 40 μl load/ cassette)
▪
500
μl loading solution (10 μl/sample)
▪
2 X 800
l 0.1% Tween 20 in running buffer*
▪
40 ml of spare running buffer
* Tween solution is used to improve DNA recovery of larger DNA fragments.
Important!
Fluorescein labels will degrade at room temperature
– minimize time at RT for
labeled standards and markers