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BluePippin Operations Manual 460013 Rev F
10-5
2.
Continuity Test will Automatically Run.
The continuity test screen will launch. The
separation and elution channel test parameters are displayed and the electrical current
results will be listed after several seconds. If the test returns a “PASS” message, press
“RETURN” and continue to baseline calibration.
Failed Continuity Tests
1.
A failed test is indicated by a “FAIL” message, and the failed channel is highlighted in
orange.
Figure 10.5
shows a failed test screen (elution channel in lane 1).
2.
If a Separation lane has failed (left column) continuity.
Do not use that lane.
Remaining passing lanes can be used if necessary.
3.
If an Elution channel (right column) has failed continuity.
Replace buffer in the
elution module of the failed lane and refill with 40 ul of fresh electrophoresis buffer and
retest the cassette. If the lane fails again, do not use the lane for collections.
Figure 10.5
. The Continuity Test screen. The elution channel in lane 1 has failed.
Test parameters
Separation Channels
“FAIL” = do not use lane
Elution Channels
“FAIL” =replace buffer and
retest. If it fails again, do not
use for collections.