PerkinElmer IVIS Lumina III Series, Hardware Manual

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IVIS ® Lumina XRMS Series III Hardware Manual Chapter 10 | Troubleshooting
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10.4 No Image Produced
If no image is produced, there may be an error in the Living Image software, a problem with the
physical connections to the camera, or a hardware failure.
1.
Close the Living Image ® software and restart the computer.
2.
Restart the Living Image software and try to acquire an image.
3.
If after restarting the computer, you are still unable to produce an image, contact PerkinElmer
Technical Support for assistance (see
page 24 ).
Table 10.1 Troubleshooting – Unacceptable Luminescent Image
Problem Corrective Action
Light contamination-
Internal Check to see that there are no extraneous light sources in the imaging chamber.
Many substances phosphoresce when exposed to light. Be especially cautious of
plastics and substances that contain pigment. Be sure to pre-screen any substance or
material before performing actual experiments.
Light contamination-
External A two inch diameter High Reflectance Hemisphere (PerkinElmer part no. 118937) is
used to help check for light leaks (
Figure 10.3 ). Contact PerkinElmer Technical
Support to purchase this accessory (see page 24 ).
To check for light leaks using the High Reflectance Hemisphere:
3.
Place the high reflectance hemisphere in the imaging chamber on the stage
using a subject height of 3.5 cm at field of view D.
4.
Take a luminescent image of the hemisphere using the luminescent settings: F/
Stop = 1, Binning = Large (high sensitivity), and exposure time = 5 minutes.
If the hemisphere can be easily seen, there is a light leak. Contact PerkinElmer
Technical Support for assistance.
Camera noise Verify that the camera is cooled to the demand temperature.
Check the measured temperature in the IVIS Acquisition Control Panel Acquisition
Control Panel to ensure that it is locked. If the camera temperature is locked, the
camera temperature box is green.
If the camera temperature box is red, click the red box to display the actual
temperature. See
Measured temperature is warmer than the demand temperature.
on page 74 .
Figure 10.3
High Reflectance Hemispheres