IVIS
®
Lumina XRMS Series III Hardware Manual
Chapter 8 | Fluorescence Module
60
The lamp intensity level is computer-controlled by the Living Image
®
software. The user can adjust
the lamp output intensity by means of software to a low or high setting. The fluorescence light source
module operates under software control; therefore manual adjustment of the front panel lamp
potentiometer is disabled.
The illumination system of the IVIS Lumina XRMS Series III is optimized for NIR imaging. The
Extended NIR range (EKE-ER) 150W tungsten excitation light source allows increased power at
wavelengths greater than 700 nm (red curve in
) compared to the previous illumination
system (blue curve in
).
The lamp output is delivered to the excitation filter wheel assembly (
) on the back of the
IVIS Lumina XRMS Series III imaging chamber.
shows a cross section of the excitation
filter wheel assembly. Light from the input fiber optic bundle passes through a collimating lens then
travels through a 25 mm diameter excitation filter. Twenty-two (22) position filter wheel locations
allow you to choose up to nineteen excitation filters. Light blocks are provided in the three unused
filter slots. One of these positions is used during bioluminescent imaging to prevent external light
from entering the imaging chamber. The 22 position excitation filter wheel is motor-controlled
through the Living Image software
Figure 8.4
Relative Spectral Radiance for the Quartz Halogen Lamp
Figure 8.5
Excitation Filter Wheel Assembly
Fiber optic cable
connector
Filter wheel motor
Connector for motor
control cable
