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PerkinElmer IVIS Lumina III Series, Hardware Manual

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PerkinElmer IVIS Lumina III Series Hardware Manual Download Page 62

8 

Fluorescence Module

About the Fluorescence Module

Installation Requirements

 on page 57

Specifications

 on page 57

Description and Theory of Operation

 on page 58

Fluorescent Imaging

 on page 63

Troubleshooting

 on page 64

Care and Maintenance of the Fluorescence Equipment

 on page 67

8.1 About the Fluorescence Module

The Fluorescence Module provides IVIS Lumina XRMS Series III with fluorescent imaging 
capability. The fluorescence equipment can be used for in vitro or in vivo applications. The sensitive 
range of the IVIS Lumina XRMS Series III CCD camera sets the wavelength range for fluorescence 
applications, which is approximately 400-950 nm. As with bioluminescent imaging, wavelengths 
greater than 600 nm are preferred for in vivo fluorescent applications due to lower absorption in 
tissue. The Living Image software controls fluorescent image acquisition, including lamp power, 
level, and filter selection. 

The IVIS Lumina Series III includes an extended Range (ER) illumination source which yields 
increased brightness in the near infrared (NIR) region, especially at wavelengths greater than 700nm. 
The excitation filter wheel has 22 positions that are filled with 19 narrow band filters spanning the 
range from 410 to 790 nm. The emission filter wheel has 8 positions with 7 broad band emission 
filters covering the range from 500 to 865 nm. A high service temperature fiber optic cable connects 
the illumination source to the input of the excitation filter wheel. 

This chapter explains how to operate the fluorescence equipment with the IVIS Lumina XRMS Series 
III. It also provides important safety and maintenance information.

You should also be familiar with and refer to the other chapters of this manual for the IVIS Lumina 
XRMS Series III. 

For instructions on how to use the Living Image software that controls fluorescent image acquisition, 
see the Living Image

®

 Software Manual for IVIS Lumina XRMS Series III that is provided with the 

software.

The Schott Fostec DCR

®

 III Direct Current Regulated Light Source User’s Manual and Technical 

Reference is a separate manual for the light source module. It provides additional useful information 
on the light source module and its safe operation.

!

CAUTION: 

Only use the fiber optic cable that is provided with the IVIS Lumina

XRMS Series III. Other fiber optic cables may be damaged by the heat from the
lamp.

IMPORTANT: 

To ensure optimum and safe performance of the Fluorescence Module

with maximum service life, read this chapter carefully before you use IVIS Lumina
XRMS Series III with the Fluorescence Module.

Summary of Contents for IVIS Lumina III Series

Page 1: ...PN CLS137706 RevB IVIS Lumina XRMS Series III December 2013 H A R D W A R E M A N U A L ...

Page 2: ...ed in the United States and or other countries Reproduction of this publication or parts thereof in any form is expressly prohibited without the express written permission of PerkinElmer Any errors or omission which may have occurred in this publication despite the utmost care taken in its production will be corrected as soon as possible but not necessarily immediately upon detection PerkinElmer p...

Page 3: ... Cleaning Liquid Entry 5 Moving the IVIS Lumina XRMS Series III 6 2 7 Power Considerations 6 Power Sources 6 Power Cord Protection 6 Lightning and Power Line Surges 6 Power Outages 6 Overloading 7 2 8 Servicing 7 2 9 Other Equipment 7 Chapter 3 Warnings 8 3 1 Electrical Safety 8 3 2 X Ray Safety 8 3 3 Eye Safety and Burn Hazard 9 3 4 Mechanical Safety 9 3 5 Chemical and Biological Safety 10 3 6 Pa...

Page 4: ...on X rays 18 Turning Off X rays 20 X ray ON Indicators 21 Safety Interlocks 21 Safety Testing 22 5 5 Your Laboratory X ray Safety Procedures 22 Contact Your Radiation Protection Authorities 22 Study Documentation 22 Create Radiation Safety Plan 22 Create a Training Plan 23 Safety Maintenance 23 Regulatory Compliance 24 5 6 Contact Information 24 PerkinElmer Sales and Technical Support 24 US State ...

Page 5: ...Module 56 8 2 Installation Requirements 57 8 3 Specifications 57 Electrical Power and Fuses 57 Environmental 57 Lamp and Fuse 57 Ventilation Requirements 57 Chemicals Required for Operation 58 Weight and Dimensions of the Fluorescence Light Source Module 58 8 4 Description and Theory of Operation 58 System Components 58 Understanding Filter Spectra 62 8 5 Fluorescent Imaging 63 IVIS Acquisition Co...

Page 6: ...0 Troubleshooting 74 10 1 Measured Temperature Is Not Equal to Demand Temperature 74 10 2 Photographic Image Is Unacceptable 75 10 3 Luminescent Image Is Unacceptable 75 10 4 No Image Produced 76 Appendix A XWS 260 Workstation 77 A 1 Shutting Down the Imaging System 78 A 2 Moving the Imaging System on the XWS 260 Workstation 78 A 3 Starting the Imaging System 78 Appendix B Options and Accessories ...

Page 7: ... 790 nm 8 position filter wheel with 7 emission filters spanning the wavelength range from 500 865 nm Living Image Software for automated image acquisition post processing and data analysis A Windows based computer system for data acquisition and analysis This manual explains how to operate and maintain the equipment and provides guidelines for obtaining optimal bioluminescent fluorescent and X ra...

Page 8: ...damage It is also used to alert you to unsafe practices It reminds you that all safety instructions should be read and understood before installation operation maintenance or repair of this instrument When you see this symbol pay particular attention to the safety information presented Observance of safety precautions will help avoid actions that could damage or adversely affect the performance of...

Page 9: ...ions Failure to follow operating or handling instructions may void any warranty covering this product Heed Warnings Abide by all warnings on the product and in the operating instructions Failure to adhere to warnings or safety precautions may void any warranty covering the IVIS Lumina XRMS Series III Table 2 1 Safety symbols Symbol Definition Warning Hazardous voltage Warning The equipment produce...

Page 10: ...untry where the equipment is operated This includes all local state and federal regulations In some states of the US it may be necessary to register radiation sources with the governing state and or local public health agencies before operating the instrument Equipment registration may be required immediately or within 30 days of acquiring the equipment Owners and operators of the IVIS Lumina XRMS...

Page 11: ...at surface of the rear panel should be provided behind the IVIS Lumina XRMS Series III to provide unobstructed air flow and access to the main power on off switch The work space is level Heat The system should be situated away from heat sources such as open flames radiators heat registers stoves and other heat generating electrical equipment Water and Moisture 2 6 Cleaning or Moving the System Com...

Page 12: ...this device to protect against electrical transient events Failure to isolate the camera from electrical transients may result in damage to the CCD camera Power Outages If the IVIS Lumina XRMS Series III experiences a loss of supply power turn off the power switch for all components and do not restart the system until reliable power has been restored CAUTION The IVIS Lumina XRMS Series III is sens...

Page 13: ...sentative voids the warranty covering the IVIS Lumina XRMS Series III 2 9 Other Equipment Use of any equipment other than that recommended by this manual has not been evaluated for safety and therefore is the sole responsibility of the user Do not modify the IVIS Lumina XRMS Series III in ANY manner by making any kind of hole or aperture in the instrument or removing any component of the radiation...

Page 14: ...enter the light source module under any circumstances See Cleaning the IVIS Lumina XRMS Series III on page 69 for instructions WARNING When the power is on DO NOT disconnect or reattach the electrical control cable that connects the fluorescence equipment excitation filter assembly to the light source module and the IVIS Lumina XRMS Series III electronics tray See System Components page 45 for pho...

Page 15: ... not be handled with bare hands or sprayed with cleaning agents WARNING DO NOT operate the light source module or the fluorescence equipment without all of the fiber optic cables connected at both of their end connections WARNING Do not attempt to replace the tungsten halogen lamp or lamp assembly in the light source module Lamp replacement requires PerkinElmer Technical Support see page 24 becaus...

Page 16: ...ou about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow Handle all infectious samples according to good laboratory procedures and methods to prevent the spread of disease 3 6 Panels Cover and Modules There are no user serviceable components in the lower electronics tray of the IVIS Lumina XRMS Series III Do not remo...

Page 17: ...System malfunction or failure during normal usage in accordance with the instructions in this manual Repairs and replacements under the Limited Warranty will be made at PerkinElmer s expense Parts replaced during this Limited Warranty period may be retained by PerkinElmer at its sole option and will be warranted for the longer of the remaining term of the original Limited Warranty period or for th...

Page 18: ... ERROR FREE d Some countries states or provinces do not allow the exclusion or limitation of implied warranties or the limitation of incidental or consequential damages for certain products or the limitation of liability for personal injury so the above limitations and exclusions may be limited in their application to you When any implied warranties are not allowed to be excluded in their entirety...

Page 19: ...our license agreement or allowed by law you may not use copy reproduce translate broadcast modify license transmit distribute exhibit perform publish or display any part in any form or by any means Reverse engineering disassembly or decompilation of this software unless required by law for interoperability is expressly prohibited b Use of the System This manual has been developed for use by proper...

Page 20: ...nformation on international limits for X ray doses see X ray Dose Limits on page 17 later in this section Other Product Documentation Users will receive pre installation information to help prepare the laboratory for installation of the IVIS Lumina XRMS Series III Included with this package of material is a document called 126198 Safe Operating and Emergency Procedures for the operation of the IVI...

Page 21: ...ielding that completely blocks scattered radiation in all possible directions including the primary beam direction Ionizing radiation can also be produced by radioactive materials but IVIS Lumina XRMS Series III contains no radioactive materials This is important because there is no possibility of transferring a radioactive contaminant into the laboratory during a procedure such as cleaning the im...

Page 22: ... occurs when an X ray photon interacts with an orbital electron and transfers energy to it causing the electron to be ejected from the atom Such ionizations may disrupt cellular molecules such as DNA A DNA molecule may be broken by the radiation and the cell may be severely damaged resulting in cell death With enough cell death tissue and organs may be damaged Injury to a living organism can also ...

Page 23: ...urface of the equipment under maximum operating conditions PerkinElmer certifies that IVIS Lumina XRMS Series III system has achieved the objectives of ICRP 60 recommendations of annual public dose limit of 100mrem ICRP 103 recommendations of annual public dose limit of 100mrem US OSHA workplace annual public dose limits of 100mrem and other international public safety standards and regulations It...

Page 24: ...witch or by turning the door handle which activated the primary interlock switch Finally a redundant interlock as prescribed by the United States FDA completely disconnects power to the X ray source when the door is opened to a gap of 7 mm 0 28 inches Because of the knife edge design of the light and X ray leakage seal no radiation could escape through this gap Also during X ray operation a soleno...

Page 25: ...f for some reason the emergency OFF switch has been push in to the off position it can be reset to ON by rotating the red knob in a counter clockwise direction Figure 5 1 Key Switch Figure 5 2 Arming Switch and Indicator Figure 5 3 Control Panel Living Image Software Click to start X ray mode Select X ray mode ...

Page 26: ...the session by pushing the computer stop button as shown in Figure 4 X rays will be stopped if the Key switch is turned OFF or if the emergency OFF switch is pushed see Figure 5 Finally any attempt to open the door will activate the primary interlock thus turning off the X rays Abnormal turning off of the x rays will require re arming the yellow push button switch on the main console control panel...

Page 27: ...image acquisition Unless the door is completely closed and the handle locked the CCD camera shutter will not open A second redundant door interlock prevents X ray generation by physically removing part of the X ray electrical circuit when the door is opened Figure 5 6 In addition to the two safety interlocks the door has a solenoid activated lock that prevents the door from being opened during an ...

Page 28: ...edures for the operation of the IVIS Lumina XRMS Series III Cabinet X ray System Pay particular heed to the safety procedures described in document 126198 This document can help with preparing a radiation safety plan described in the next section Also the Living Image Software Manual for IVIS Lumina XRMS Series III provides the basic operating instructions for bioluminescence fluorescence and X ra...

Page 29: ...survey techniques if surveys are not conducted by a PerkinElmer representative Methods of controlling radiation dose such as time distance and shielding The principles and practice of maintaining X ray exposure to as Low As Reasonably Achievable ALARA Personal monitoring of X ray exposure Symptoms of acute localized exposure and proper reporting procedure for an actual or suspected exposure Applic...

Page 30: ...stallation instructions and is also included on the same CD ROM as this manual International customers should check with their governing bodies about possible registration or other requirements PerkinElmer certifies that the IVIS Lumina XRMS Series III meets the Japanese standard JAIMAS0101 2001 with respect to X ray containment PerkinElmer certifies that the IVIS Lumina XRMS Series III complies w...

Page 31: ...61 2301 www healthyarkansas com rtl CALIFORNIA Radiologic Health Branch Division of Food and Radiation Safety PO Box 997414 MS 7610 Sacramento CA 95899 7414 916 440 7899 http www cdph ca gov programs Pages RadiologicHealthBranch aspx COLORADO X Ray and Mammography Compliance Registration and Certification 4300 Cherry Creek Drive South Denver CO 80246 1530 303 692 3446 http www cdphe state co us hm...

Page 32: ...5 4540 www doh state fl us environment radiation GEORGIA Department of Human Resources 2 Peachtree Street NW 33rd Floor Atlanta GA 30303 3142 404 657 5400 http ors dhr georgia gov portal site DHR ORS HAWAII Dept of Health Noise and Radiation Branch 591 Ala Moana Blvd Honolulu HI 96813 4921 808 586 4700 Radiation Section Radiological Response Radiologic Technology Mammography 808 586 4700 http hawa...

Page 33: ...90 Ext 257 http www in gov isdh 23279 htm IOWA Bureau of Radiological Health Lucas State Office Bldg 5th Fl 321 E 12th St Des Moines IA 50309 4611 515 281 3478 http www idph state ia us eh radiological_health asp KANSAS Radiation Section 1000 SW Jackson St Suite 310 Topeka KS 66612 1366 785 296 1565 http www kdheks gov radiation indexXray html KENTUCKY Radiation Control Program Cabinet for Health ...

Page 34: ...f Public Health Schrafft Center Suite 1M2A 529 Main Street Charlestown MA 02129 617 242 3035 www mass gov dph rcp MICHIGAN Radiation Safety Section Div of Health Facilities and Services Bureau of Health Systems MI Dept of Community Health PO Box 30664 Lansing MI 48909 517 241 1993 www michigan gov rss MINNESOTA Section of Indoor Environments and Radiation Division of Environmental Health Departmen...

Page 35: ...f Health and Human Services P O Box 95026 Lincoln NE 68509 5026 402 471 0528 www dhhs ne gov rad NEVADA Radiological Health Section Bureau of Health Protection Services Nevada State Health Division 4510 Technology Way Suite 300 Carson City NV 89706 775 687 7540 http health nv gov HCQC_Radiological htm NEW HAMPSHIRE Radiological Health Section Division of Public Health Services Dept of Health and H...

Page 36: ...tt Drive Raleigh NC 27609 7221 919 571 4141 Ext 232 http www ncradiation net NORTH DAKOTA Division of Air Quality North Dakota Dept of Health 918 E Divide Avenue Bismarck ND 58501 1947 701 328 5188 www ndhealth gov aq rad OHIO Bureau of Radiation Protection Ohio Dept of Health 246 North High Street Columbus OH 43215 614 644 2727 http www odh ohio gov odhPrograms rp radprot radprot1 aspx OKLAHOMA C...

Page 37: ...e RI 02908 5097 401 222 4520 http www health ri gov hsr facilities radiological index php SOUTH CAROLINA Bureau of Radiological Health Dept of Health and Environment Control 2600 Bull Street Columbia SC 29201 803 545 4420 http www scdhec gov health radhlth x ray htm SOUTH DAKOTA Office of Health Care Facilities Licensure and Certification 615 East 4th St Pierre SD 57501 1700 605 773 3356 http doh ...

Page 38: ...alth James Madison Bldg 109 Governor Street Room 730 Richmond VA 23219 804 864 8170 http www vdh state va us epidemiology RadiologicalHealth WASHINGTON Office of Radiation Protection Department of Health PO Box 47827 Olympia WA 98504 7827 360 236 3210 http www doh wa gov ehp rp xray reg htm WEST VIRGINIA Radiological Health Program Office of Environmental Health Services DHHR Bureau for Public Hea...

Page 39: ... 10808 99th Ave 8th Floor Edmonton AB T5K 0G5 Tel 780 415 0612 Fax 780 422 0014 British Columbia Radiation Protection Services BC Centre for Disease Control 655 12th Avenue West Vancouver BC V5Z 4R4 Tel 604 660 6630 Fax 604 660 6628 Department of National Defence Director General Nuclear Safety Department of National Defence Rm 1702 Standard Life Building 280 Slater Street Ottawa ON K1A 0K2 Tel 61...

Page 40: ... Services West Block 4th Fl Confederation Bldg PO Box 8700 St John s NL A1B 4J6 Tel 709 729 0218 Fax 709 729 3445 Northwest Territories Workers Safety and Compensation Commission Northwest Territories and Nunavut PO Box 8888 Yellowknife NT X1A 2R3 Tel 867 669 4407 or 1 800 661 0792 Fax 867 873 0262 Nova Scotia Occupational Health and Safety Division Department of Labour and Workforce Development P...

Page 41: ... Quebec Direction de la prevention Inspection Commission de la sante et de la securite du travail 524 rue Bourdages local 250 CP 1200 succursale Terminus Quebec QC G1K 7E2 Contact Mrs Candide Fournier Tél 418 266 4699 ext 2005 Saskatchewan Radiation Safety Unit Department of Labour 400 1870 Albert St Regina SK S4P 4W1 Tel 306 787 4538 Fax 306 787 2208 Yukon Workers Compensation Health and Safety 4...

Page 42: ... coupled device CCD camera which can image animal subjects using three modalities bioluminescence fluorescence and X ray IVIS Lumina XRMS Series III includes A camera An imaging chamber with controlling electronics An X ray source and detector including controls and safety systems A Fluorescence Module including excitation and emission filters The system is controlled by a pre configured computer ...

Page 43: ... photographic VOLTAGE IVIS Lumina XRMS Series III can operate at multiple voltages 100 240 VAC However users are not permitted to change the input voltage to any of the system components Several internal modifications are required for voltage change If you need to change the operating voltage contact PerkinElmer Technical Support see page 24 IMPORTANT If you modify the IVIS Lumina XRMS Series III ...

Page 44: ...r imaging modalities including photographic imaging Specific information including care of the scintillation plate is given in the next section The X ray system schematic is shown in Figure 6 2 A solenoid driven X ray filter is located near the X ray source The filter screens out unwanted X ray energies from the total radiation spectrum The filter s normal position is over the source but it can be...

Page 45: ...ition For subject height up to 2 8 cm Upper position For subject height up to 5 3 cm See page 49 for instructions on changing the scintillation plate position After X ray imaging the screen rotates out the way under a protective cover Figure 6 3 The cover prevents incidental afterglow from interfering with other imaging modalities and protects the screen window from contamination for example clean...

Page 46: ...ber switch has been pushed and the light is ON indicating that all safety interlocks are functioning properly The X ray source cannot be energized from the Living Image software until these conditions have been fulfilled CAUTION DO NOT direct strong sprays of cleaning liquids at the scintillation module While reasonably water tight a directed spray could penetrate the seals of the module and damag...

Page 47: ... archive the spare key If the keys are lost contact PerkinElmer Technical Support see page 24 6 6 Imaging Chamber The imaging chamber is a highly specialized device consisting of the imaging chamber housing a heated movable platform an auto focusing lens system with F Stop control a filter wheel and sample illumination LEDs Figure 6 5 All adjustable components are motorized and computer controlled...

Page 48: ...aging Chamber Specifications 6 7 Optics 6 8 Optical Filter Wheel A 22 position excitation filter wheel with 19 equally spaced narrow band filters is attached to the back of the imaging chamber Table 6 2 An 8 position computer controlled optical filter wheel is located at the top of the imaging chamber in front of the imaging lens The filter wheel settings are selected in the Living Image software ...

Page 49: ...atures High speed Windows based PC Microsoft Windows family operating system Living Image software installed This software controls the IVIS Lumina XRMS and displays and analyzes image data CD burner installed for data storage and transport Network ready 24 high resolution flat screen monitor for image viewing Microsoft Office installed Table 6 2 IVIS Lumina XRMS Series III Filters Excitation Filt...

Page 50: ...17 5 cm W Weight 27 56 lbs 12 5 Kg Computer Monitor Flat screen Description Power requirements 0 6 A at 120 V 0 35 A at 240 V 50 60 Hz Dimensions with stand 10 13 D x 15 5 H x 22 03 W 25 7 cm D x 39 48 cm H x 55 96 cm W Weight with stand 14 7 lbs 6 67 Kg Environmental Requirements Specification Temperature 15 C to 25 C 50 F to 78 F Humidity 0 to 80 non condensing Type of use Indoor Imaging chamber...

Page 51: ...ce lamp are also turned to the On position 5 Start the Living Image software after the desktop screen is displayed 6 Enter a User ID up to three letters when prompted then click Done 7 Click Initialize in the IVIS Acquisition Control Panel Figure 7 2 Allow the system to initialize You will hear the motors move The System Status box displays the current changes The temperature square in the IVIS Ac...

Page 52: ...off the power switch on each component Do not restart the system until reliable line power has been restored 1 Turn on the computer 2 Turn on the imaging chamber 3 Start the Living Image software and click Initialize IVIS System in the IVIS Acquisition Control Panel 4 For X ray mode the system will need to be re armed by pressing the amber arming switch on the front panel 7 3 Gas Plumbing Anesthes...

Page 53: ...a XRMS Series III DO NOT USE FLAMMABLE ANESTHESIA GAS CAUTION It is recommended to use the XGI 8 Gas Anesthesia System when imaging small animals Figure 7 4 The system supplies a controlled amount of isoflurane to the imaging chamber and continuously reduces the build up of isoflurane in the chamber If you want to use a gas other than the recommended isoflurane oxygen gas mixture or pure air conta...

Page 54: ...stage curtain attached to the IVIS imaging chamber platform covers the empty space beneath the platform Figure 7 5 The stage curtain serves as a reminder to not place anything below the platform The curtain attaches to the platform by a bar that is held in place by clips at two locations If it is necessary to access this area the curtain can be easily removed and replaced To release the rod and cu...

Page 55: ...e in Lower Position NOTE Changing the scintillation plate position is a manual process Table 7 1 Choosing the Scintillation Plate Position Subject Scintillation Plate Position To Move the Scintillation Plate Rat Upper position for vertical subject height up to 5 3 cm Put a check mark next to Large Animal option in the control panel Follow the instructions for Moving the Scintillation Plate to the ...

Page 56: ...e scintillation plate out by pulling the plate toward you The magnets and alignment pins on the scintillation plate are visible after the plate is removed from the scintillation assembly The scintillation assembly has matching magnets and pin receptacles Figure 7 9 Figure 7 7 Control Panel Figure 7 8 Remove the Locking Screw Software prompt Locking screw Scintillation plate ...

Page 57: ...Remove the cover by pulling the alignment pins of the cover out of the scintillation assembly c Place the cover in the upper position using the alignment pins as guides d Tighten the thumb screw Figure 7 9 Alignment Pins and Magnets Figure 7 10 Placing the Scintillation Plate in Upper Position Alignment pins and magnets Magnets upper position Magnets lower position Right alignment pin receptacle u...

Page 58: ...ve the scintillation plate to the lower position 1 Remove the check mark next to Large Animal in the control panel Figure 7 7 The scintillator assembly moves to the service position The software prompts you to move the scintillator assembly to the lower position 2 Remove the locking screw using the hex wrench provided with the imaging system Figure 7 7 Figure 7 11 Moving the Scintillation Plate Co...

Page 59: ...page 51 4 Place the scintillation plate in the lower position using the alignment pins and magnets as guides Figure 7 14 5 Reinstall the locking screw 6 Move the scintillation plate cover to the lower position a Loosen the thumb screw Figure 7 15 b Remove the cover by pulling it off the alignment pins c Place the cover in the lower position using the alignment pins as guides d Tighten the thumb sc...

Page 60: ...ot using the FOV mat supplied as an accessory to the Lumina XRMS during imaging in the X ray mode where high resolution is desired The FOV mat material permits more X ray scatter Some compromise may be necessary however if the X ray acquisition is coupled with fluorescence imaging In this case the low auto fluorescence image produced with the FOV mat material may be preferred to the X ray image wi...

Page 61: ...MS Series III turning the system components on and off If it is necessary to shut down the imaging system for any reason it is important to follow the procedure below 1 Close the Living Image software and save any information of interest at the prompt 2 Return the X ray ON key selector switch to the OFF position and remove the key 3 Turn off the IVIS Lumina XRMS Series III imaging chamber using th...

Page 62: ...nd filters spanning the range from 410 to 790 nm The emission filter wheel has 8 positions with 7 broad band emission filters covering the range from 500 to 865 nm A high service temperature fiber optic cable connects the illumination source to the input of the excitation filter wheel This chapter explains how to operate the fluorescence equipment with the IVIS Lumina XRMS Series III It also provi...

Page 63: ...ll as adequate room for fiber optic cable routing IMPORTANT The fluorescence equipment operates at the same voltage as the IVIS Lumina XRMS Series III chamber and must not be used at other than its labeled voltage Voltage Available 90 260 VAC 50 60 Hz Power Consumption 150 W NOTE See Chapter 6 on page 36 for more information on additional electrical power requirements of the IVIS Lumina XRMS Serie...

Page 64: ...ption and Theory of Operation System Components The Fluorescence Module provides fluorescent imaging capability You can conveniently switch between bioluminescent and fluorescent imaging applications Figure 8 1 shows the fluorescence equipment and Figure 8 2 depicts the interior of the imaging chamber green indicates the excitation light Weight 4 94 lbs 2 24 kg Depth 8 61 inches 21 9 cm Width 7 27...

Page 65: ...umina XRMS wavelength range of 400 950 nm The EKE ER lamp provides improved light output in the 400 950nm range compared to the standard EKE lamp Because of the higher temperature of the ER lamps a high temperature fiber optic is used to deliver the light to the excitation filter wheel Figure 8 2 IVIS Lumina XRMS Series III and Fluorescence Equipment Figure 8 3 Fluorescence Light Source Module Exc...

Page 66: ...lamp output is delivered to the excitation filter wheel assembly Figure 8 5 on the back of the IVIS Lumina XRMS Series III imaging chamber Figure 8 6 shows a cross section of the excitation filter wheel assembly Light from the input fiber optic bundle passes through a collimating lens then travels through a 25 mm diameter excitation filter Twenty two 22 position filter wheel locations allow you to...

Page 67: ...all the stage locations are peaked near their center The non uniformity of the illumination pattern is compensated for when units of efficiency are selected in the Living Image software for more details see the Living Image Software User s Manual When imaging 96 well plates the lower stage positions C and D are recommended to minimize shadowing effects due to the off axis illumination Fluorescent ...

Page 68: ... its central wavelength and its width at 50 peak transmission full width half maximum FWHM Real filters have some leakage outside of the bandpass region and can also exhibit autofluorescence depending on the materials used in the filter construction More realistic filter transmission curves are shown in Figure 8 9 Figure 8 8 Typical Excitation and Emission Spectra for a Fluorescent Compound Figure...

Page 69: ...e IVIS Acquisition Control Panel Figure 8 10 To acquire a fluorescent image the user must check the Fluorescent box on the left side of the panel Once selected controls for the illumination lamp Fluor Lamp Level and Filter Lock will appear in the top half of the panel Checking the Filter Lock box ensures that the excitation and emission filters are properly paired During image acquisition the lamp...

Page 70: ...ly brighter than bioluminescence so the exposure time is shorter and F Stop higher smaller lens opening Typical fluorescence image camera settings might be 1 sec exposure time Binning Medium and F Stop 2 10 Click Acquire After the exposure is completed the overlay image is displayed 11 To save the data select Living Image Save Living Image Data from the main menu bar This completes the data acquis...

Page 71: ...he AC line cord from the power entry module on the rear panel of the light source module 5 Open the fuse holder door The fuse holder is part of the power entry module and is located directly below where the AC line cord plugs in Figure 8 11 Use a thin bladed screwdriver or penknife blade if necessary Be careful not to damage the drawer WARNING DO NOT disconnect or reattach the electrical control c...

Page 72: ...switch to the ON 1 position the red indicator will be visible on the switch 11 Turn on the main power switch at the rear of the IVIS Lumina XRMS Series III and start the Living Image software 12 Resume normal operation Lamp Replacement Contact PerkinElmer Technical Service see page 24 for lamp replacement Replacing the lamp requires re calibration of the IVIS Lumina XRMS Series III The procedure a...

Page 73: ...ut cleaning or sterilizing any of the optical components or the optical filter replacement see page 24 WARNING DO NOT use fluids to clean the exterior or interior of the IVIS Lumina XRMS Series III Do not allow fluids of any kind to enter the light source module under any circumstances Sprays and liquids that come into contact with the light source module or the instrument may result in damage to ...

Page 74: ...radiation leakage test is complex and requires sensitive equipment Some states or localities may require special training and certification to perform the test Contact PerkinElmer Technical Support see page 24 for information regarding these tests or for scheduling a PerkinElmer representative to conduct the survey as part of an overall safety check 9 2 Maintenance and Safety Checks Daily Safety C...

Page 75: ... source and restart X ray generation from the Living Image software Annual Safety Checks Perform the following safety checks every 12 months All safety checks performed on a daily weekly and monthly basis A full radiation survey performed by a qualified person 9 3 Cleaning the IVIS Lumina XRMS Series III The compounds shown in Table 9 1 do not damage the internal finish of the IVIS Lumina XRMS Ser...

Page 76: ... Cleaning the Scintillation Plate Window and Holder The scintillation plate is protected by a glass window with anti reflection coating This window may be cleaned using the optical cleaner and soft absorbent wipes provided in the Scintillation Plate Maintenance Kit Window cleaning may be accomplished without removing the scintillation plate holder but rotating it to the center of the stage enables...

Page 77: ... a small portion of the soft lint free wipe PerkinElmer part no 126291 with the optical cleaner PerkinElmer part no 123495 Figure 9 3 Figure 9 1 Gas Manifold Connections Figure 9 2 Scintillation Plate Moved to Center of the Stage Gas tubing connected at the right hand elbow on the gas manifold Gas tubing connected at the barbed fitting on the stage Gas manifold gas hose disconnected from the right...

Page 78: ...soft wipe in ethyl alcohol and rub the underside of the holder It may be convenient to use a foam paint applicator to support the alcohol soaked wipe Figure 9 5 Support the applicator with the wipe as shown in Figure 9 5 to prevent bending the plate holder A solution of 3 to 5 bleach may be used on the underside of the plate holder if care is used not to get it on the AR coated window Figure 9 3 A...

Page 79: ... Support the Alcohol soaked Lint free Wipe with a Foam Paint Applicator NOTE If this method does not completely clean the plate holder it may be necessary to remove the plate holder from the drive mechanism for cleaning See Appendix C Scintillation Plate Holder on page 81 for instructions ...

Page 80: ...ng Image Software Table 10 1 Troubleshooting Measured Temperature Does Not Equal the Demand Temperature Problem Possible Cause Corrective Action Measured temperature is warmer than the demand temperature Ambient temperature may be too high camera air vents my be blocked or system needs service A problem may exist with the camera Verify that the room temperature is within operational limits Check a...

Page 81: ...ng Unacceptable Photographic Image Problem Possible Cause Corrective Action Image is streaked Subject moved during the exposure Check to see if the subject may have moved If the subject is not on the sample stage it is probably on the floor of the imaging chamber If the subject has moved locate and re anesthetize it If gas anesthesia is being used confirm that the anesthesia is turned on and the f...

Page 82: ...n External A two inch diameter High Reflectance Hemisphere PerkinElmer part no 118937 is used to help check for light leaks Figure 10 3 Contact PerkinElmer Technical Support to purchase this accessory see page 24 To check for light leaks using the High Reflectance Hemisphere 3 Place the high reflectance hemisphere in the imaging chamber on the stage using a subject height of 3 5 cm at field of vie...

Page 83: ...er Move the workstation with the components Restart the system components after the power is restored CAUTION PerkinElmer recommends that you do not move an IVIS Lumina XRMS Series III that is not located on the XWS 260 workstation If you need to move an imaging system not on the workstation contact PerkinElmer Technical Support for assistance see page 24 CAUTION The IVIS Lumina XRMS Series III ha...

Page 84: ...power surge protection devices 3 Turn on the computer and monitor 4 Turn on the IVIS Lumina XRMS Series III imaging chamber the power switch is located on the back of the unit and verify that the other components such as the camera power supply X ray controller both on the back of the unit and fluorescence lamp are also turned to the On position 5 Start the Living Image software after the desktop ...

Page 85: ...ntrol panel Figure A 2 For operating instructions see the Living Image Software Manual for IVIS Lumina XRMS Series III 9 To use the X ray modality a Verify that the Emergency OFF switch is in the ON position b Turn the key selector switch to the ON position c Confirm that the amber arming switch has been pushed and the light is ON d Verify that the X ray source is armed e Use the IVIS Acquisition ...

Page 86: ...ssories 125698 XRH 2 Reflective Hemisphere 118937 XPP 1 Plate Positioner 118949 XPM 2 Bioluminescent Phantom Mouse 118993 XFM 2 Fluorescent Phantom Mouse 121365 XAF 8 Anesthesia System Filters 118999 XFM 1 Low Fluorescence Mat set of 10 119000 XNC 2 Mouse Nose Cones set of 10 119001 XRS 10 Rubber Stoppers for Anesthesia Manifold 119006 XWS 260 Workbench 123325 XWS 248 Workbench 123326 XWS 272 Work...

Page 87: ...conducting this procedure If screws are lost inside the imaging chamber you should try to recover them to prevent damage to the mechanisms under the stage C 1 Removing the Scintillation Plate Holder 1 Close the Living Image software and turn off the power to the IVIS Lumina XRMS Series III at the main power switch that is located at the rear of the instrument near the power cord 2 Disconnect the m...

Page 88: ...intillation plate holder a Remove the 9 16 Hex screw Figure C 2 Two pins will hold the scintillator in position after the Hex screw is removed b Pull the scintillator out The scintillation plate holder and its window can now be cleaned See Section 9 4 on page 70 for cleaning instructions CAUTION Avoid putting fingerprints on the window Pull straight forward without bending the plate holder up or d...

Page 89: ...or cover by horizontally rotating the plate holder 90 degrees clockwise Figure C 4 4 Close the imaging chamber door and turn on the power to the Lumina XRMS at the rear main power switch 5 Re initialize the instrument through the Living Image software This moves the plate holder to the home position by locating the limit sensors Figure C 3 Insert Scintillation Plate Holder on L Bracket Figure C 4 ...

Page 90: ...F filter lock 63 filter replacement 67 filter spectra 62 fluorescence equipment See Fluorescence Module Fluorescence Module installation requirements 57 system components 58 fluorescent imaging 63 64 fuse 57 fuse replacement 65 66 G gas plumbing 46 glowing materials 55 H hardware problems 64 hazardous voltage symbol 3 hazards 9 heat safety 5 humidity 57 I imaging chamber 41 42 door 48 gas plumbing...

Page 91: ...sition 52 upper position 49 servicing 7 servicing the equipment 8 shut down procedure 55 specifications computer 44 computer monitor 44 electrical power 57 environmental 57 fuse 57 humidity 57 imaging chamber 42 lamp 57 optics 42 temperature 57 stage curtain 48 start up 78 79 restarting after power outage 46 subject placement 54 system cleaning 69 70 environmental requirements 44 moving with XWS 2...

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