OPTIMedical OPTI CCA-TS, Operator'S Manual, Page: 160 / 271

Operator’s Manual – OPTI CCA-TS 8-5
8 OPERATING PRINCIPLES
Erythrocyte aggregation and sedimentaton may occur very quickly in syringes containing pathologic
blood samples and may adversely affect the measurement of ctHb in any analyzer. To prevent such errors,
first insert the OPTI CCA-TS cassette into the analyzer to initiate calibration. Next, mix the syringe
sample well by rolling the syringe for at least 60 seconds, after expelling any trapped bubbles, then
immediately measure in the OPTI CCA-TS.
The OPTI CCA-TS system aspirates blood in the same manner from syringes, capillaries or
ComfortSampler.
No changes are made to the aspiration rate, volume or timing. Therefore, there are no biases or
imprecision dependent upon the sample introduction method. Sufficient volume must, however, be
present in syringes (0.25 mL in a 1 mL syringe) to prevent mechanical interference between the syringe
plunger and the syringe adapter.
Errors in blood analysis on properly collected samples may result from improper mixing of the sample
after collection and before measurement; contamination with room air resulting from failure to expel any
trapped bubbles after collection; and from metabolic changes in the sample.
Serum samples should be obtained by collecting blood in an untreated blood collecting tube. The sample
should stand for 30 minutes to allow the clot to form prior to centrifugation. After centrifugation, remove
the serum from the clot, and cap or seal the sample tube. If storage is required, the sample should be
tightly capped, refrigerated at 4 to 8 °C for no longer than 48 hours, and allowed to return to room
temperature, 15 to 30 °C, prior to analysis. Each laboratory should determine the acceptability of its own
blood collection syringes, capillaries and tubes and the serum or plasma separation products.
Variations in these products exist between manufacturers, and at times, from lot to lot.
NOTE: Serum is an unsuitable sample material for accurate glucose analysis, because the retention
time of the erythrocytes in the sample is too long. The process of glycolysis may lead to
decreased glucose values in serum samples.
8.5 Procedure
8.5.1 Materials Needed
Description Part Number
Sensor Cassettes see Chapter 9, Supplies
in various analyte configurations
Standard Reference Cassette Level 1 BP7536
Standard Reference Cassette Level 3 BP7543
Calibration Gas Bottle BP7001
Printer Paper HP0070
The OPTI CCA-TS automatically processes the sample through the necessary steps, then displays and
prints the results. For details of this operation, please refer to Chapter 5 of the Operator’s Manual.