Molecular Devices FlexStation II, Operator'S Manual

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3.5 Configuring Instrument Settings in Flex Mode
3.5.12 Compound and Tip Columns
The choices in this window depend upon the number of transfers chosen in the
Compound Transfer window. When one or more transfers are enabled, these settings
allow you to choose the tips and compounds that will be used for transfers.
The settings in this window are either automatically assigned by the software or you can
manually assign them into any configuration you require.
Compound & Tip Columns Automatically Assigned
SOFTmax PRO automatically enters information in the Compound & Tip Columns
window taking into consideration the number of transfers, the Wells to Read section,
and the Pipette Tips Layout selected area already selected. The software assumes fluid
will be aspirated from the compound plate starting with the first available column
indicated in the Pipette Tip Layout. Aspirated fluid will then be dispensed to the first
available column indicated in the Wells to Read selection.
The software assumes:
•
All columns in the Wells to Read selection should receive fluid;
•
The fluids are transferred from left to right; the read-transfer-read sequence in each
column is initiated only after the previous column’s read-transfer-read event is
completed (the total read time for that column);
•
The fluid transfer targets are cumulative from transfer to transfer (that is, the second
transfer’s targets start with the next available clean tip and untargeted compound
column rather than reusing tips and compound columns targeted by the preceding
fluid transfer); and
•
Each fluid transfer will use a new tip.
The software assumes that all of the columns in the Wells to Read area will receive fluid
during the initial transfer. The Tips target grid will be filled left to right starting with the
first available tip column and incrementing the Tip target by one until all columns in the
Wells to Read selection have been filled.
FlexStation II Operator’s Manual – Rev. D 69